Characterization of VIP- and helodermin-preferring receptors on human small cell lung carcinoma cell lines.

Abstract

We investigated the molecular and pharmacologic characteristics of VIP receptors on two human SCLC cell lines: NCI-N592 and NCI-H345. With NCI-N592 cell, the order of potency of VIP-related peptides in inhibiting 125I-VIP binding and in stimulating cAMP production was typical of the human VIP receptor. By covalent cross-linking, a polypeptide of Mr 62,300 was obtained. Conversely, the behavior of NCI-H345 cell line was totally different: helodermin was the most potent peptide, VIP and PHI were equipotent, while hGRF and secretin were totally ineffective. These results suggest that NCI-N592 cells possess a typical VIP receptor while NCI-H345 cells possess a helodermin-preferring receptor, and that the natural target of helodermin might not be the VIP receptor.

• Authors: J Luis, S I Said
• Journal: Peptides (Peptides) 1990 Nov-Dec Vol. 11 Issue 6 Pg. 1239-44 ISSN: 0196-9781 [Print] United States
• PMID: 1965034 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Cross-Linking Reagents
• Intercellular Signaling Peptides and Proteins
• Iodine Radioisotopes
• Peptides
• Receptors, Cell Surface
• Receptors, Gastrointestinal Hormone
• Receptors, Neuropeptide
• Receptors, Vasoactive Intestinal Peptide
• helodermin receptor
• Vasoactive Intestinal Peptide
• heliodermin
• Cyclic AMP

Topics

• Carcinoma, Small Cell (metabolism)
• Cell Membrane (metabolism)
• Cross-Linking Reagents
• Cyclic AMP (metabolism)
• Electrophoresis, Polyacrylamide Gel
• Humans
• Intercellular Signaling Peptides and Proteins
• Iodine Radioisotopes
• Lung Neoplasms (metabolism)
• Peptides
• Receptors, Cell Surface (chemistry)
• Receptors, Gastrointestinal Hormone (chemistry, metabolism)
• Receptors, Neuropeptide
• Receptors, Vasoactive Intestinal Peptide
• Tumor Cells, Cultured
• Vasoactive Intestinal Peptide (metabolism)