Potentiation of the antiviral activity of poly r(A-U) by xanthene dyes.

Abstract

Ten xanthene dyes (XAN) are evaluated for their ability to potentiate the antiviral activity of poly r(A-U) using a human foreskin fibroblast-vesicular stomatitis virus bioassay in which the XAN is combined with 0.2 mM poly r(A-U) to produce a XAN/ribonucleotide ratio of 1/4. Four of the ten XANs tested in this study, rhodamine 123, rhodamine B, rhodamine 6G and sulforhodamine B, enhance the antiviral activity of poly r(A-U) 8- to 15-fold. The interferon-inducing activity of the four active XAN/poly r(A-U) combinations is equal to the sum of the activities of their constituents. These four XANs appear to potentiate the antiviral activity of the poly r(A-U) without superinduction of interferon. The direct viral inactivation study demonstrates that the XANs, poly r(A-U) and the XAN/poly r(A-U) combinations do not inactivate the VSV at concentrations near the 50% effective dose.

• Authors: J M Jamison, K Krabill, A Hatwalkar, E Jamison, C C Tsai
• Journal: Cell biology international reports (Cell Biol Int Rep) Vol. 14 Issue 12 Pg. 1075-84 (Dec 1990) ISSN: 0309-1651 [Print] England
• PMID: 1964628 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antiviral Agents
• Coloring Agents
• Interferon Inducers
• Rhodamines
• Xanthenes
• Poly A-U

Topics

• Antiviral Agents (pharmacology)
• Cells, Cultured
• Coloring Agents (pharmacology)
• Drug Synergism
• Fibroblasts (drug effects, metabolism)
• Humans
• Interferon Inducers (pharmacology)
• Male
• Molecular Structure
• Poly A-U (pharmacology)
• Rhodamines (pharmacology)
• Structure-Activity Relationship
• Vesicular stomatitis Indiana virus (physiology)
• Virus Replication (drug effects)
• Xanthenes (pharmacology)