[Interest of monoclonal antibodies in biomedical laboratory analysis].

Abstract

Immunoassays, or assays with antibodies as reagents, are widely used in medical laboratories. These assays are used to identify and quantify various substances in biological fluids, such as specific proteins (various tissue markers, markers of inflammation, hormones, coagulation factors...) or immunoglobulins (viral or bacterial antibodies, auto-antibodies...) and even both viral antigens and antibodies (HIV virology). The use of monoclonal antibodies allowed, through their specificity for a single epitope of the target molecule, the development of increasingly sophisticated immunoassays. In particular, the use of monoclonal antibodies with microarrays permits the simultaneous determination of various proteins (inflammatory profile, cardiac profile, specifics IgE...) quickly and accurately. Very important tools in the clinical laboratory, immunoassays techniques are, however, subject to various analytical interferences which may be responsible for significant changes in the test results.

• Authors: V I Mistretta, E Cavalier, J Collette, L Lutteri, J P Chapelle
• Journal: Revue medicale de Liege (Rev Med Liege) 2009 May-Jun Vol. 64 Issue 5-6 Pg. 257-63 ISSN: 0370-629X [Print] Belgium
• Vernacular Title: Intérêt des anticorps monoclonaux dans le laboratoire d'analyses biomédicales.
• PMID: 19642455 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antibodies, Monoclonal

Topics

• Antibodies, Monoclonal (analysis)
• Antibody Specificity
• Humans
• Immunoassay