Abstract | INTRODUCTION: METHODS: The aim of the current in vitro study was to evaluate the effect of the intensity (1-20 mg/l) and duration (1, 2, 7 or 14 days) of ganciclovir exposure on toxicity in B lymphoblastoid cells (using cell counting and viability measurements). RESULTS: A correlation was found between the dose of ganciclovir exposure and a decrease in total cell number when duration exceeded 2 days (r(2)=0.92 and 0.93 after 7 and 14 days, respectively). High levels (20 mg/l) of ganciclovir were not more toxic than lowest levels (1 mg/l) for the shortest durations of ganciclovir exposure (1 and 2 days). Moreover, 50% cytotoxic concentrations markedly decreased with the duration of ganciclovir exposure (374-3 mg/l from 1 to 14 days respectively) after 14 days of culture. CONCLUSIONS: This in vitro study demonstrated for the first time that ganciclovir exhibited an in vitro duration-dependent toxicity on haematopoietic-derived cells when in vivo doses of the drug were used.
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Authors | Audrey Janoly-Dumenil, Isabelle Rouvet, Nathalie Bleyzac, Yves Bertrand, Gilles Aulagner, Marie-Thérèse Zabot |
Journal | Antiviral chemistry & chemotherapy
(Antivir Chem Chemother)
Vol. 19
Issue 6
Pg. 257-62
( 2009)
ISSN: 2040-2066 [Electronic] England |
PMID | 19641234
(Publication Type: Journal Article)
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Chemical References |
- Antiviral Agents
- Ganciclovir
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Topics |
- Antiviral Agents
(toxicity)
- B-Lymphocytes
(cytology, drug effects)
- Bone Marrow
(drug effects)
- Cell Line
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Child
- Dose-Response Relationship, Drug
- Ganciclovir
(toxicity)
- Humans
- Time Factors
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