IL-18 serum level and IL-18 promoter gene polymorphism in Iranian patients with gastrointestinal cancers.

Abstract

BACKGROUND AND AIM:

Interleukin (IL)-18 level and association of its two promoter gene polymorphisms at -607 C/A and -137 G/C positions were investigated in Iranian patients with gastrointestinal (GI) cancers.

METHODS:

232 cases of GI cancers and 312 healthy controls were enrolled. Serum level of IL-18 was measured by enzyme linked immunosorbent assay (ELISA) and genotyping of IL-18 gene polymorphisms were assessed by allele-specific polymerase chain reaction (PCR).

RESULTS:

There was a significant difference in the frequency of -137 G/C genotype between patients with stomach or colorectal cancers and control group. In patients with colorectal cancer, the frequency of the -607 AA/-137 GC genotype combination in unwell-differentiated cases was more than those with well-differentiated cancer. Haplotype analysis showed that in patients with stomach cancer -607 C/-137 C and -607 A/-137 G and in patients with colorectal cancer -607 C/-137 C were decreased compared with control group, and this difference reached statistical significance. Serum analysis revealed that the mean IL-18 serum level in stomach and colorectal cancer before and after surgical operation was significantly higher than healthy volunteers. Postoperative IL-18 level for all patients with colorectal cancer was significantly decreased compared with the levels before surgery.

CONCLUSION:

Results of this investigation suggests that Single Nucleotide Polymorphism (SNP) at position -137 G/C and haplotype frequency may play a role in predisposition of Iranian patients to stomach and colorectal cancers. In addition, increasing serum IL-18 level may have clinical importance as a diagnostic marker in patients with stomach and colorectal cancer.

• Authors: Mohammad Reza Haghshenas, Seyed Vahid Hosseini, Mahmoud Mahmoudi, Mehdi Saberi-Firozi, Shirin Farjadian, Abbas Ghaderi
• Journal: Journal of gastroenterology and hepatology (J Gastroenterol Hepatol) Vol. 24 Issue 6 Pg. 1119-22 (Jun 2009) ISSN: 1440-1746 [Electronic] Australia
• PMID: 19638090 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Interleukin-18

Topics

• Alleles
• Biomarkers (blood)
• Case-Control Studies
• Enzyme-Linked Immunosorbent Assay
• Female
• Gastrointestinal Neoplasms (blood, genetics)
• Genetic Predisposition to Disease
• Genotype
• Haplotypes
• Humans
• Interleukin-18 (blood, genetics)
• Iran
• Male
• Middle Aged
• Polymerase Chain Reaction
• Polymorphism, Single Nucleotide
• Promoter Regions, Genetic