In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines.

Abstract

In this study, we present in vitro cytotoxicity of iron oxide (Fe(3)O(4)) and manganese oxide (MnO) using live/dead cell assay, lactate dehydrogenase assay, and reactive oxygen species detection with variation of the concentration of nanoparticles (5-500 microg/ml), incubation time (18-96 h), and different human cell lines (lung adenocarcinoma, breast cancer cells, and glioblastoma cells). The surface of nanoparticles is modified with polyethyleneglycol-derivatized phospholipid to enhance the biocompatibility, water-solubility, and stability under an aqueous media. While the cytotoxic effect was negligible for 18 h incubation even at highest concentration of 500 microg/ml, MnO nanoparticle represented higher level of toxicity than those of Fe(3)O(4) and the commercial medical contrast reagent, Feridex after 2 and 4 day incubation time. However, the cytotoxicity of Fe(3)O(4) is equivalent or better than Feridex based on the live/dead cell viability assay. The engineered MnO and Fe(3)O(4) exhibited excellent stability compared with Feridex for a prolonged incubation time.

• Authors: Jong Young Choi, Su Hee Lee, Hyon Bin Na, Kwangjin An, Taeghwan Hyeon, Tae Seok Seo
• Journal: Bioprocess and biosystems engineering (Bioprocess Biosyst Eng) Vol. 33 Issue 1 Pg. 21-30 (Jan 2010) ISSN: 1615-7605 [Electronic] Germany
• PMID: 19636592 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ferric Compounds
• Manganese Compounds
• Oxides
• ferric oxide
• manganese oxide

Topics

• Biological Assay (methods)
• Cell Culture Techniques
• Cell Line, Tumor
• Dose-Response Relationship, Drug
• Ferric Compounds (adverse effects)
• Humans
• Manganese Compounds (adverse effects)
• Materials Testing (methods)
• Nanoparticles (adverse effects)
• Oxides (adverse effects)
• Time Factors