| Abstract | This study hypothesized that the expression of apoptosis-regulatory genes, such as BCL-2 and BAX may be affected by genetic variation in bleomycin-induced pulmonary fibrosis in C57BL/6 and NMRI mice. Pulmonary fibrosis induced by single intratracheal dose of bleomycin (3 U kg(-1)). After 2 weeks, lung samples were analyzed for collagen deposition, pathological changes and expression of BCL-2 and BAX. The fibrotic lung changes were similar in both strains. The immunohistochemical assay using a biotin-streptavidin technique showed no significant difference in immunoreactivity for BCL-2 protein between the controls and bleomycin-treated C57BL/6 mice. However, in NMRI mice, the expression of BCL-2 was significantly (p<0.05) upregulated in myofibroblasts and neutrophils. The expression of BAX protein was significantly (p<0.05) upregulated in alveolar epithelial cells of both strains and downregulated in myofibroblasts and lymphocytes of the lung tissues of C57BL/6 mice and also in lymphocytes of NMRI mice at 2 weeks after bleomycin instillation. These results confirm the role of BCL-2 and BAX proteins in the pathogenesis of pulmonary fibrosis and suggest that the expression of apoptotic regulatory genes may be specific in different cell types in various strains. |
| Authors | L Safaeian, A Jafarian, M Rabbani, H M Sadeghi, N Torabinia, S A Alavi
(Affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Hezar Jarib Avenue, Isfahan, Iran.)
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| Journal | Pakistan journal of biological sciences: PJBS
(Pak J Biol Sci)
Vol. 11
Issue 23
Pg. 2606-12
(Dec 1 2008)
ISSN: 1028-8880 [Print] Pakistan |
| PMID | 19630211
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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| Chemical References |
- Proto-Oncogene Proteins c-bcl-2
- bcl-2-Associated X Protein
- Bleomycin
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| Topics |
- Animals
- Bleomycin
- Gene Expression Regulation
(drug effects)
- Genetic Variation
- Immunohistochemistry
- Mice
- Mice, Inbred C57BL
- Mice, Inbred Strains
- Proto-Oncogene Proteins c-bcl-2
(genetics, metabolism)
- Pulmonary Fibrosis
(chemically induced, pathology)
- Species Specificity
- bcl-2-Associated X Protein
(genetics, metabolism)
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