[MMP-9 expression profile in inflammatory cells of Mip-1alpha knockout mice and Mip-1alpha receptor knockout mice].

Abstract

OBJECTIVE:

To explore the MMP-9 expression profile in peritoneal inflammatory macrophages and granulocytes in Mip-1alpha-, CCR1- and CCR5-deficient mice.

METHODS:

In sodium thioglycolate-induced murine peritonitis models, peritoneal macrophages and granulocytes were harvested, identified and purified from WT mice and Mip-1alpha-, CCR1-, CCR5-deficient mice. The RT-PCR was applied to evaluate the expression of MMP-9 in macrophages and granulocytes of different group of mice.

RESULTS:

The expressions of MMP-9 of macrophages in Mip-1alpha-, CCR1-, CCR5-deficient mice were significantly lower than that of WT mice (P<0.05); MMP-9 expression of granulocytes in Mip-1alpha-, CCR5-deficient mice were also significantly lower than that of WT mice (P<0.05), while the MMP-9 expression of granulocytes in CCR1-deficient mice was significant higher than that of WT mice.

CONCLUSION:

Deletion of Mip-1alpha and CCR5 could reduce the MMP-9 expression in both macrophages and granulocytes, while deletion of CCR1 could reduce MMP-9 expression in macrophages but increase MMP-9 expression in granulocytes.

• Authors: Yu Wu, Naofumi Mukaida, Ting Liu, Yong-qian Jia, Huan-ling Zhu, Yi-ming Yang, Cai-gang Xu, Zhang-xue Hu
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 40 Issue 3 Pg. 374-7 (May 2009) ISSN: 1672-173X [Print] China
• PMID: 19626984 (Publication Type: Journal Article)

Chemical References

• Chemokine CCL3
• RNA, Messenger
• Receptors, CCR1
• Receptors, CCR5
• Matrix Metalloproteinase 9
• Mmp9 protein, mouse

Topics

• Animals
• Chemokine CCL3 (genetics)
• Female
• Granulocytes (metabolism)
• Macrophages, Peritoneal (metabolism)
• Matrix Metalloproteinase 9 (genetics, metabolism)
• Mice
• Mice, Inbred BALB C
• Mice, Knockout
• RNA, Messenger (genetics, metabolism)
• Receptors, CCR1 (genetics)
• Receptors, CCR5 (genetics)