Immuno-histochemical expression of p53 protein and iNOS in odontogenic cysts.

Abstract

OBJECTIVE:

The present study investigated the expression of p53 protein and iNOS in odontogenic keratocysts (OKCs), dentigerous cysts (DCs) and radicular cysts (RCs) and determined the correlation of these 2 proteins within each cyst type.

MATERIAL AND METHOD:

p53 and iNOS in epithelial lining cells were analyzed in 20 OKCs, 20 DCs and 20 RCs using immuno-histochemistry. The percentage of positive cells was assessed and presented as mean +/-SD. The correlation between the expressions of these 2 proteins in each cyst was carried out using Spearman correlation analysis.

RESULTS:

p53 was found in 95% of OKCs, 50% of DCs and 65% of RCs. p53-positive cells in OKCs (12.2 +/- 8.8%) were significantly higher than those in DCs (1.4 +/- 3.2%) and RCs (1.3 +/- 2.4%). iNOS was found in all examined lesions. Statistically, iNOS-positive cells in RCs (86.9 +/- 9.9%) were lower than those in OKCs (94.8 +/- 3.5%) and DCs (96.1 +/- 4.4%). No correlation between the expression of p53 and iNOS was found in each cyst.

CONCLUSION:

p53 was over expressed in OKCs compared with other lesions. RCs showed the lowest expression of iNOS.

• Authors: Sopee Poomsawat, Jirapa Punyasingh, Paisarn Vejchapipat
• Journal: Journal of the Medical Association of Thailand = Chotmaihet thangphaet (J Med Assoc Thai) Vol. 92 Issue 7 Pg. 952-60 (Jul 2009) ISSN: 0125-2208 [Print] Thailand
• PMID: 19626816 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Tumor Suppressor Protein p53
• Nitric Oxide Synthase Type II

Topics

• Dentigerous Cyst (metabolism)
• Humans
• Immunohistochemistry
• Nitric Oxide Synthase Type II (metabolism)
• Odontogenic Cysts (metabolism)
• Radicular Cyst (metabolism)
• Tumor Suppressor Protein p53 (metabolism)