[Dracorhodin perchlorate inhibit high glucose-induced connective tissue growth factor formation in human mesangial cells].

Abstract

OBJECTIVE:

To study the effect of dracorhodin perchlorate on high glucose-induced connective tissue growth factor (CTGF) expression in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).

METHOD:

The HMC were divided into low glucose group (LG group, 5.5 mmol x L(-1) D-glucose), high glucose group (HG Group 25 mmol x L(-1) D-glucose), each group was located with dracorhodin perchlorate (7. 5 micromol x L(-1)) as comparison, and was examined at 24, 48 h. The expression of CTGF mRNA was assessed by semi quantatiue RT-PCR, and the expression of CTGF protein was assessed by Western blot.

RESULT:

Compared to HG group, the expression of CTGF mRNA and protein were reduced in LG group after stimulating 24, 48 hours (P < 0.01). Compared to HG group, the expression of CTGF mRNA and protein were reduced in HG group by added with dracorhodin perchlorate after stimulating 24, 48 hours (P < 0.01).

CONCLUSION:

Dracorhodin perchlorate can inhibit high glucose-induced connective tissue growth factor expression in human mesangial cells, and this may be its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).

• Authors: Yonghui Wang, Quansheng Wang, Jianguo Liu, Lixiao Zhang, Bing Zhang
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 34 Issue 7 Pg. 896-9 (Apr 2009) ISSN: 1001-5302 [Print] China
• PMID: 19623991 (Publication Type: English Abstract, Journal Article)

Chemical References

• Benzopyrans
• RNA, Messenger
• Connective Tissue Growth Factor
• dracorhodin
• Glucose

Topics

• Benzopyrans (pharmacology)
• Connective Tissue Growth Factor (genetics, metabolism)
• Dose-Response Relationship, Drug
• Gene Expression Regulation (drug effects)
• Glucose (pharmacology)
• Humans
• Mesangial Cells (drug effects, metabolism)
• RNA, Messenger (genetics, metabolism)
• Time Factors