[Effect of calmodulin antagonist O-4-ethoxyl- butyl-berbamine on the proliferation of human breast cancer cell line MCF-7 and its relevant mechanism].

Abstract

OBJECTIVE:

To investigate the effect of calmodulin antagonist O-(4-ethoxyl-butyl)-berbamine (EBB) on proliferation of human breast cancer cell MCF-7 and its possible mechanism.

METHODS:

MTT assay was used to analyze the effect of EBB on tumor cells growth. Flow cytometry was used to detect its impact on the cell cycle of MCF-7 cells. Immunofluoresce labeling technique and laser scanning confocal microscope were used to reveal the changes of the microtubule, microfilament, mitochondrion, and endoplasmic reticulum in the cells.

RESULTS:

The IC50 value of EBB in MCF-7 cells was (13.0 +/- 3.7) micromol/L. MCF-7 cells were arrested at S phase after EBB treatment. Meanwhile, depolymerization of the microtubule and microfilament, impairment of the mitochondrion and swelling of endoplasmic reticulum were observed.

CONCLUSION:

EBB arrests MCF-7 cells at S phase by inhibiting the growth of MCF-7 cells, which may be related to the changes of structures and functions of the microtubule, microfilament, mitochondrion, and endoplasmic reticulum.

• Authors: Xiao-Hua Dai, Rong Liu, Yuan Zhou, Ming Yang, Dong-Mei Fan, Dong-Sheng Xiong, Jing Qi, Chun-Zheng Yang, Hui-Fang Zhu
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 31 Issue 3 Pg. 326-9 (Jun 2009) ISSN: 1000-503X [Print] China
• PMID: 19621518 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzylisoquinolines
• Calmodulin
• O-(4-ethoxybutyl)berbamine

Topics

• Benzylisoquinolines (pharmacology)
• Breast Neoplasms (pathology)
• Calmodulin (antagonists & inhibitors)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Female
• Humans