Rapid preliminary differentiation of species within the Mycobacterium tuberculosis complex: proposition of a radiometric method.

Abstract

A radiometric method using the "Bactec 460-TB" apparatus now enables rapid obtaining of drug-susceptibility data and differentiation of clinical mycobacterial specimens into those belonging to the Mycobacterium tuberculosis complex (comprised of M. tuberculosis, M. bovis, M. bovis BCG and M. africanum) and non-tuberculous mycobacteria, using a specific inhibitor (p-nitro-alpha-acetyl-amino-beta-hydroxypropiophenone). In the present work, further differentiation of the M. tuberculosis complex into 4 individual species was achieved using the susceptibility of strains to 1.25 micrograms/ml of thiophene-2-carboxylic acid hydrazide, 40 micrograms/ml of D-cycloserine, and 100 micrograms/ml of pyrazinamide.

• Authors: K S Goh, N Rastogi
• Journal: Research in microbiology (Res Microbiol) 1991 Jul-Aug Vol. 142 Issue 6 Pg. 659-65 ISSN: 0923-2508 [Print] France
• PMID: 1961977 (Publication Type: Journal Article, Review)

Chemical References

• Thiophenes
• Hydroxypropiophenone
• thiophene-2-carboxylic acid hydrazide
• Pyrazinamide
• 4-nitro-alpha-acetylamino-beta-hydroxypropiophenone
• Cycloserine

Topics

• Cycloserine (pharmacology)
• Humans
• Hydroxypropiophenone (analogs & derivatives, pharmacology)
• In Vitro Techniques
• Mycobacterium (drug effects, isolation & purification)
• Mycobacterium tuberculosis (drug effects, isolation & purification)
• Pyrazinamide (pharmacology)
• Radiometry (methods)
• Thiophenes (pharmacology)
• Tuberculosis (microbiology)