Abstract | BACKGROUND:
Hepatocellular carcinoma (HCC) is the fifth most common malignancy in the world and constitutes the leading cause of cancer-related death among men, and second among women in Taiwan. Liver cirrhosis and HCC are relatively prevalent, and 80% to 85% of the patients with these conditions have positive results for hepatitis B surface antigen in Taiwan. Only 5% of the general population is seronegative for all hepatititis B virus (HBV) markers. This is the first study to determine the role of ezrin upon HBV HCC cell and patients with HBV HCC undergoing hepatectomy
METHODS: Immunohistochemical study with ezrin in 104 human HBV-HCC cases were carried out to investigate its association with the clinicopathological features and the outcomes of 104 HBV-HCC patients undergoing hepatetomy. In addition, DNA constructs including the wild type ezrin (wt- ezrin) and mutant ezrin Tyr353 (Y353) were transfected into Hep3B cell to study its role in tumor invasion and differentiation. RESULTS: HBV HCC patients with ezrin over-expression independently have smaller tumor size, cirrhotic liver background, poor tumor differentiation, and more vascular invasion. Ezrin expression status has no impact on survival for HBV-HCC patients undergoing hepatectomy. The in vitro assay showed that wt- ezrin Hep3B cells have a significant higher level of AFP secretion and higher invasion ability as compared with the control and Y353- ezrin Hep3B cells. CONCLUSION:
Ezrin over-expression contributed to de-differentiation and invasion of HBV-HCC cell. HBV-HCC patients with ezrin over-expression were independently associated with tumor with smaller size, cirrhotic liver background, poor differentiation, and vascular invasion.
|
Authors | Chun-Nan Yeh, See-Tong Pang, Tsung-Wen Chen, Ren-Ching Wu, Wen-Hui Weng, Miin-Fu Chen |
Journal | BMC cancer
(BMC Cancer)
Vol. 9
Pg. 233
(Jul 15 2009)
ISSN: 1471-2407 [Electronic] England |
PMID | 19604375
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Cytoskeletal Proteins
- ezrin
|
Topics |
- Adult
- Aged
- Carcinoma, Hepatocellular
(complications, metabolism)
- Cell Differentiation
- Cytoskeletal Proteins
(biosynthesis)
- Female
- Gene Expression Regulation, Neoplastic
- Gene Expression Regulation, Viral
- Hepatectomy
- Hepatitis B
(complications, metabolism)
- Humans
- Liver Neoplasms
(complications, metabolism)
- Male
- Middle Aged
- Mutation
- Neoplasm Invasiveness
- Treatment Outcome
|