Abstract | BACKGROUND:
Shikonin derivatives have cytotoxic and antitumor effects. This study aims to investigate the antitumor effects of acetylshikonin isolated from a Chinese medicinal herb Arnebia euchroma (Royle) Johnst. METHODS: RESULTS: In A549, Bel-7402, MCF-7 and LLC cell lines, acetylshikonin inhibited cell growth in a dose-dependent manner. IC50 (means +/- SD) were 5.6 +/- 0.86 microg/ml, 6.82 +/- 1.5 microg/ml, 3.04 +/- 0.44 microg/ml and 2.72 +/- 0.38 microg/ml respectively. Acetylshikonin suppressed tumor growth in C57BL/6 mice with LLC. The inhibition rate of acetylshikonin (2 mg/kg) was 42.85%. Immunohistochemical staining revealed that in the acetylshikonin groups the expression of bax and caspase-3 increased, whereas the expression of bcl-2 decreased, suggesting that acetylshikonin induced tumor cell apoptosis through activating the pro-apoptotic bcl-2 family and caspase-3. CONCLUSION:
Acetylshikonin isolated from Arnebia euchroma (Royle) Johnst cell suspension cultures exhibits specific in vivo and in vitro antitumor effects.
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Authors | Wenbi Xiong, Gang Luo, Liming Zhou, Yun Zeng, Wenji Yang |
Journal | Chinese medicine
(Chin Med)
Vol. 4
Pg. 14
(Jul 11 2009)
ISSN: 1749-8546 [Electronic] England |
PMID | 19594888
(Publication Type: Journal Article)
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