The design, synthesis and evaluation of hypoxia-activated pro-oligonucleotides.

Abstract

Hypoxia-activated pro-oligonucleotides were synthesized through the commercial phosphoramidite method, and could be readily cleaved to form normal oligos with good hypoxia selectivity in vitro under the effect of reductases, as well as in tumor cell extract.

• Authors: Nan Zhang, Chunyan Tan, Puqin Cai, Peizhuo Zhang, Yufen Zhao, Yuyang Jiang
• Journal: Chemical communications (Cambridge, England) (Chem Commun (Camb)) Issue 22 Pg. 3216-8 (Jun 14 2009) ISSN: 1359-7345 [Print] England
• PMID: 19587918 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oligonucleotides
• Prodrugs
• Nitroreductases
• Fluorescein

Topics

• Animals
• Base Sequence
• Cattle
• Drug Design
• Escherichia coli (enzymology)
• Fluorescein (metabolism)
• HeLa Cells
• Humans
• Hypoxia
• Nitroreductases (metabolism)
• Oligonucleotides (chemical synthesis, chemistry, genetics, metabolism)
• Prodrugs (chemical synthesis, chemistry, metabolism)
• Staining and Labeling