The protective efficacy of magnolol in hind limb ischemia-reperfusion injury.

Abstract

We investigated the protective effects of magnolol, an active antioxidant and free radical scavenger extracted from Magnolia officinalis, in a hind limb ischemic-reperfusion animal model. Adult male Sprague-Dawley rats were subjected to hind limb ischemic insult for 2 hours and were intravenously treated with magnolol at 0.01 mg/kg (n=8), 0.3 mg/kg (n=8) mg/kg or 1 mg/kg (n=8) mg/kg, or vehicle (n=8). At 24 h post-insult, the levels of nitrite/nitrate (NOX), malondialdehyde (MDA) and myeloperoxidase (MPO), as well as the degree of muscle damage, were assessed. Relative to controls, animals treated with magnolol (0.3 and 1 mg/kg) had attenuated muscular inflammation, edema and damage. Magnolol (0.3-1 mg/kg) also effectively reduced postischemic rises in the MDA, NOx and MPO levels (p<0.05, respectively). Magnolol administrated at 0.01 mg/kg, however, failed to protect against the ischemic-perfusion limb injury. In addition, magnolol (0.01-1 mg/kg) did not affect local muscular blood reperfusion or other physiological parameters, including hematocrit, glucose, arterial blood gases and mean arterial blood pressure. Thus, intravenous administration with magnolol at 0.3-1 mg/kg protects against ischemic limb damage in rats. This cytoprotection may be attributed to its antioxidant, anti-nitrosative and anti-inflammatory actions.

• Authors: Hung-Yi Chen, Yu-Chang Hung, E-Jian Lee, Tsung-Ying Chen, I-Chuan Chuang, Tian-Shung Wu
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 16 Issue 10 Pg. 976-81 (Oct 2009) ISSN: 1618-095X [Electronic] Germany
• PMID: 19577912 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Biphenyl Compounds
• Lignans
• Plant Extracts
• magnolol
• Nitric Oxide
• Malondialdehyde
• Peroxidase

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology, therapeutic use)
• Biphenyl Compounds (pharmacology, therapeutic use)
• Body Temperature (drug effects)
• Drug Evaluation, Preclinical
• Hindlimb (blood supply, enzymology, pathology)
• Lignans (pharmacology, therapeutic use)
• Magnolia (chemistry)
• Male
• Malondialdehyde (metabolism)
• Nitric Oxide (blood)
• Peroxidase (metabolism)
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Regional Blood Flow (drug effects)
• Reperfusion Injury (enzymology, pathology, prevention & control)