Cancer chemopreventive activity of diversin from Ferula diversivittata in vitro and in vivo.

Abstract

A prenylated coumarin (diversin, 1) together with four new sesquiterpene lactones (diversolides A, D, F and G, 2-5) isolated from the roots of Ferula diversivittata were studied for their possible inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). All of the tested compounds were active against EBV-EA activation. Among these compounds diversin (IC(50): 7.7) exhibited the strongest inhibitory effect and was selected to examine its effects on in vivo two-stage mouse skin carcinogenesis induced by 7,12-dimethylbenz[a]anthracene (DMBA) as initiator and TPA as promoter. Treatment with compound 1 (85nmol) along with DMBA/TPA inhibited papilloma formation up to week 7 and the percentage of papilloma bearers was approximately 93.3% at week 20. The average number of papillomas formed per mouse was only 5.5 even at week 20. The results of the present investigation indicated that diversin might be valuable as a potent cancer chemopreventive agent and its potency was comparable with those of curcumin and quercetin, two well-known cancer chemopreventive agents.

• Authors: M Iranshahi, A Sahebkar, S T Hosseini, M Takasaki, T Konoshima, H Tokuda
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 17 Issue 3-4 Pg. 269-73 (Mar 2010) ISSN: 1618-095X [Electronic] Germany
• PMID: 19577457 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 Elsevier GmbH. All rights reserved.

Chemical References

• Antigens, Viral
• Antineoplastic Agents, Phytogenic
• Carcinogens
• Coumarins
• Epstein-Barr virus early antigen
• Lactones
• Monoterpenes
• Plant Extracts
• Sesquiterpenes
• diversin
• 9,10-Dimethyl-1,2-benzanthracene
• Tetradecanoylphorbol Acetate

Topics

• 9,10-Dimethyl-1,2-benzanthracene
• Animals
• Antigens, Viral (metabolism)
• Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology, therapeutic use)
• Carcinogens
• Coumarins (isolation & purification, pharmacology, therapeutic use)
• Female
• Ferula (chemistry)
• Lactones (isolation & purification, pharmacology, therapeutic use)
• Mice
• Mice, Inbred ICR
• Monoterpenes (isolation & purification, pharmacology, therapeutic use)
• Papilloma (pathology, prevention & control)
• Phytotherapy
• Plant Extracts (chemistry, pharmacology, therapeutic use)
• Plant Roots
• Sesquiterpenes (isolation & purification, pharmacology, therapeutic use)
• Skin (drug effects, pathology)
• Skin Neoplasms (pathology, prevention & control)
• Tetradecanoylphorbol Acetate