Efficacy of arbidol on lethal hantaan virus infections in suckling mice and in vitro.

Abstract

AIM:

Arbidol is an immunomodulator that was first developed in Russia. In this study, we report the antiviral activity of arbidol against Hantaan virus (HTNV) in vitro and in vivo.

METHODS:

The antiviral activity of arbidol in vitro was determined by plaque-forming assay, ranging from 0.5 to 8 microg/mL. To investigate whether arbidol has an antiviral effect in vivo, suckling BALB/c mice infected with HTNV were treated with arbidol at 24 h before infection with a 5, 10 or 20 mg.kg(-1).d(-1), once per day, for 10 days. On day 12 and 28 post infection (pi), histopathological changes and viral antigen were detected. On days 4, 8, 12, and 16 pi, the viral load of target organs and serum TNF-alpha levels of arbidol-treated animals were determined.

RESULTS:

Arbidol was found to have potent inhibitory activity against HTNV when added in vitro before or after viral infection, with a 50% inhibitory concentration (IC(50)) of 0.9 and 1.2 microg/mL, respectively. The 50% lethal dose (LD(50)) of arbidol for suckling mice was 78.42 mg.kg(-1).d(-1). Oral administration of arbidol increased both survival rate and mean time to death (MTD). Treatment with arbidol reduced histopathological changes, decreased viral load and viral antigen levels, and modulated the level of serum TNF-alpha.

CONCLUSION:

Arbidol has the ability to elicit protective antiviral activity against HTNV in vivo and in vitro.Acta Pharmacologica Sinica (2009) 30: 1015-1024; doi: 10.1038/aps.2009.53; published online 8 June 2009.

• Authors: Hai-Ying Deng, Fan Luo, Li-Qiao Shi, Qiong Zhong, Ying-Juan Liu, Zhan-Qiu Yang
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 30 Issue 7 Pg. 1015-24 (Jul 2009) ISSN: 1745-7254 [Electronic] United States
• PMID: 19575005 (Publication Type: Journal Article)

Chemical References

• Antiviral Agents
• Immunologic Factors
• Indoles
• Placebos
• Tumor Necrosis Factor-alpha
• umifenovir

Topics

• Animals
• Animals, Newborn
• Animals, Suckling (virology)
• Antiviral Agents (therapeutic use)
• Brain (pathology, virology)
• Cell Line
• Female
• Hantaan virus (drug effects)
• Hemorrhagic Fever with Renal Syndrome (drug therapy, pathology, veterinary)
• Humans
• Immunologic Factors (therapeutic use)
• Indoles (pharmacology, therapeutic use)
• Kidney (pathology, virology)
• Lethal Dose 50
• Lung (pathology, virology)
• Mice
• Mice, Inbred BALB C
• Placebos
• Pregnancy
• Random Allocation
• Tumor Necrosis Factor-alpha (blood)