Antigenic proteins of Lactobacillus acidophilus that are recognised by serum IgG antibodies in children with type 1 diabetes and coeliac disease.

Abstract

Immune responses to lactobacilli have been so far insufficiently investigated in patients with autoimmune diseases. We used whole-cell lysate of an indigenous Lactobacillus acidophilus strain isolated from an Estonian child to study serum IgG antibodies in children groups with type 1 diabetes [insulin dependent diabetes mellitus (IDDM)] (n = 21, age 4-18 yr) and with acute coeliac disease (CD) (n = 20, age 0.6-15 yr) and to compare the results with the controls (n = 24, age 2-17 yr). We found that our developed 1-D immunoblot assay readily enables to reveal antibodies against 28 L. acidophilus antigenic proteins in patients' and controls' sera. As verified by immunoproteomics analysis with 2-D and LC ESI-MS/MS the antigens of L. acidophilus were mainly common cytoplasmic proteins GroEL (HSP60), enolase, transcription factor EF-Ts and EF-Tu. However, in addition we identified formyl-CoA transferase being target for antibodies in every tested IDDM patients' serum. We have characterized for the first time the antigenic profile of L. acidophilus whole-cell lysate using sera from children with IDDM, CD, and controls. The different prevalence of reactions against tested antigens in patients and controls sera may indicate significant differences in immune system and commensal bacteria cross-talk in these groups.

• Authors: Anna-Liisa Prangli, Meeme Utt, Ija Talja, Epp Sepp, Marika Mikelsaar, Tarvo Rajasalu, Oivi Uibo, Vallo Tillmann, Raivo Uibo
• Journal: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology (Pediatr Allergy Immunol) Vol. 21 Issue 4 Pt 2 Pg. e772-9 (Jun 2010) ISSN: 1399-3038 [Electronic] England
• PMID: 19573144 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Epitopes, B-Lymphocyte
• Immunoglobulin G
• Coenzyme A-Transferases
• formyl-coenzyme A transferase

Topics

• Adolescent
• Antigens, Bacterial (immunology)
• Celiac Disease
• Child
• Child, Preschool
• Coenzyme A-Transferases (immunology)
• Diabetes Mellitus, Type 1 (blood, immunology)
• Epitopes, B-Lymphocyte (metabolism)
• Female
• Humans
• Immunoglobulin G (blood)
• Infant
• Lactobacillus acidophilus (immunology)
• Male
• Mass Spectrometry