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Cyclic alphavbeta6-targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma.

AbstractBACKGROUND:
A cyclic peptide-displaying phage library was used for biopanning on oral squamous cell carcinoma (OSCC) cells to identify cancer-targeting peptides. This study was designed to characterize the receptor specificity of a candidate phage clone/peptide (phage/peptide-29) and to explore the clinical potential of this peptide.
METHODS:
Immunofluorescent confocal microscopy, phage binding assay, and immunohistochemical studies were used to demonstrate the receptor specificity of phage/peptide-29. The effect of peptide-29 on the proliferation of OSCC cells was studied using 3-dimensional (3D) cell cultures.
RESULTS:
Phage/peptide-29 preferentially binds integrin alphavbeta6 rather than other alphav-associated integrins. Peptide-29 significantly inhibits the proliferation of OSCC cells in 3D cell cultures. On human pathological sections, phage-29 targets oral cancer cells in a alphavbeta6-dependent manner. Besides, we showed that integrin alphavbeta6 is universally (94.7%, 36/38) expressed in all major kinds of head and neck squamous cell carcinomas (HNSCC).
CONCLUSIONS:
Peptide-29 selected from biopanning may have clinical potential for HNSCC.
AuthorsJenn-Ren Hsiao, Yao Chang, Yuh-Ling Chen, Su-Huei Hsieh, Keng-Fu Hsu, Chun-Fu Wang, Sen-Tien Tsai, Ying-Tai Jin
JournalHead & neck (Head Neck) Vol. 32 Issue 2 Pg. 160-72 (Feb 2010) ISSN: 1097-0347 [Electronic] United States
PMID19572290 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2009 Wiley Periodicals, Inc.
Chemical References
  • Antigens, Neoplasm
  • Integrins
  • Peptide Library
  • Peptides
  • integrin alphavbeta6
Topics
  • Antigens, Neoplasm (metabolism)
  • Carcinoma, Squamous Cell (metabolism)
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Proliferation
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunohistochemistry
  • Integrins (metabolism)
  • Microscopy, Confocal
  • Mouth Neoplasms (metabolism)
  • Peptide Library
  • Peptides (metabolism)

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