Anti-clastogenic effect of magnolol-containing Hange-koboku-to, Dai-joki-to, Goshaku-san, and Magnoliae Cortex on benzo(a)pyrene-induced clastogenicity in mice.

Abstract

Magnolol was previously shown to inhibit genotoxicity induced by environmental mutagens both in vitro and in vivo. Here, we investigate the effects of the magnolol-containing kampo (traditional) medicines Hange-koboku-to, Dai-joki-to, and Goshaku-san, as well as Magnoliae Cortex, on the clastogenesis induced by benzo(a)pyrene (B(a)P) using the mouse micronucleus test. The mice were first treated with a single intraperitoneal injection of B(a)P, followed by a single oral dose of Hange-koboku-to, Dai-joki-to, Goshaku-san, or Magnoliae Cortex. Peripheral blood specimens were prepared 48 h after B(a)P administration and analyzed using the acridine orange (AO) technique. The anti-clastogenic mechanisms employed by the kampo medicines and Magnoliae Cortex were also investigated by evaluating in vivo CYP1A1 activity using the zoxazolamine paralysis test. Results show that Hange-koboku-to, Dai-joki-to, and Magnoliae Cortex, which contain high levels of magnolol, significantly inhibited the clastogenesis induced by B(a)P and sufficiently inhibited in vivo CYP1A1 activity. In contrast, Goshaku-san, which contains low levels of magnolol, had little inhibitory effect on clastogenicity and in vivo CYP1A1 activity. These findings suggest that magnolol is a major contributor to the inhibition of B(a)P-induced clastogenesis, and that kampo medicines exert significant anti-clastogenic effects.

• Authors: Junichiro Saito, Hiroko Fukushima, Hisamitsu Nagase
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 32 Issue 7 Pg. 1209-14 (Jul 2009) ISSN: 0918-6158 [Print] Japan
• PMID: 19571387 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimutagenic Agents
• Biphenyl Compounds
• Drugs, Chinese Herbal
• Lignans
• Mutagens
• magnolol
• Benzo(a)pyrene
• Cytochrome P-450 CYP1A1

Topics

• Animals
• Antimutagenic Agents (isolation & purification, pharmacology)
• Benzo(a)pyrene (toxicity)
• Biphenyl Compounds (isolation & purification, pharmacology)
• Cytochrome P-450 CYP1A1 (antagonists & inhibitors)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (isolation & purification, pharmacology)
• Lignans (isolation & purification, pharmacology)
• Magnolia (chemistry)
• Medicine, Kampo
• Mice
• Mice, Inbred ICR
• Micronucleus Tests
• Mutagens (toxicity)
• Plant Bark (chemistry)