The relationship between cell membrane damage and lipid peroxidation under the condition of hypoxia-reoxygenation: analysis of the mechanism using antioxidants and electron transport inhibitors.

Abstract

We consecutively observed lipid peroxidation and cell membrane damage under the condition of hypoxia-reoxygenation (H/R) in cells and analyzed their mechanisms by using electron transport inhibitors and an antioxidant. In H/R experiments, lipid peroxidation and cell membrane damage were observed during the hypoxia phase. In the reoxygenation phase, lipid peroxidation stopped, while cell membrane damage did not. An antioxidant, n-acetylcystein (NAC), and potassium cyanide (KCN) inhibited lipid peroxidation and cell membrane damage, while rotenone did not inhibit either of them. Although antimycin A did not inhibit lipid peroxidation, it inhibited cell membrane damage during the hypoxia phase but not during the reoxygenation phase. These results suggested that lipid peroxidation can affect cell membrane damage as a trigger during the hypoxia phase and the generation of oxidative stress can vary depending on the inhibition locations in the electron transport system.

• Authors: Daisuke Yajima, Hisako Motani, Mutsumi Hayakawa, Yayoi Sato, Kaoru Sato, Hirotaro Iwase
• Journal: Cell biochemistry and function (Cell Biochem Funct) Vol. 27 Issue 6 Pg. 338-43 (Aug 2009) ISSN: 1099-0844 [Electronic] England
• PMID: 19569086 (Publication Type: Journal Article)
• Copyright: (c) 2009 John Wiley & Sons, Ltd.

Chemical References

• Antioxidants
• Fluorescent Dyes
• Reactive Oxygen Species
• Oxygen

Topics

• Animals
• Antioxidants (pharmacology)
• Cell Death
• Cell Hypoxia
• Cell Membrane (metabolism)
• Cell Membrane Permeability (drug effects, physiology)
• Cells, Cultured
• Electron Transport (drug effects, physiology)
• Fluorescent Dyes
• Lipid Peroxidation (drug effects, physiology)
• Mice
• Oxygen (physiology)
• Reactive Oxygen Species (metabolism)
• Reperfusion Injury (metabolism)
• Time Factors