The effect of self-assembling peptide RADA16-I on the growth of human leukemia cells in vitro and in nude mice.

Abstract

Nanofiber scaffolds formed by self-assembling peptide RADA16-I have been used for the study of cell proliferation to mimic an extracellular matrix. In this study, we investigated the effect of RADA16-I on the growth of human leukemia cells in vitro and in nude mice. Self-assembly assessment showed that RADA16-I molecules have excellent self-assembling ability to form stable nanofibers. MTT assay displayed that RADA16-I has no cytotoxicity for leukemia cells and human umbilical vein endothelial cells (HUVECs) in vitro. However, RADA16-I inhibited the growth of K562 tumors in nude mice. Furthermore, we found RADA16-I inhibited vascular tube-formation by HUVECs in vitro. Our data suggested that nanofiber scaffolds formed by RADA16-I could change tumor microenvironments, and inhibit the growth of tumors. The study helps to encourage further design of self-assembling systems for cancer therapy.

• Authors: Chengkang Tang, Ximing Shao, Binbin Sun, Wenli Huang, Xiaojun Zhao
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 10 Issue 5 Pg. 2136-2145 (May 14 2009) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 19564944 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peptides
• RADA16-I

Topics

• Animals
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Heterografts
• Human Umbilical Vein Endothelial Cells
• Humans
• Hydrophobic and Hydrophilic Interactions
• Jurkat Cells
• Leukemia
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Microscopy, Atomic Force
• Nanofibers
• Neoplasm Transplantation
• Peptides (metabolism, toxicity)
• Tumor Microenvironment (drug effects)