Abstract |
Clinical and experimental data show an increase in sodium reabsorption on the proximal tubule (PT) in essential hypertension. It is well known that there is a link between essential hypertension and renal angiotensin II (Ang II). The present study was designed to examine ouabain-insensitive Na(+)-ATPase activity and its regulation by Ang II in spontaneously hypertensive rats (SHR). We observed that Na(+)-ATPase activity was enhanced in 14-week-old but not in 6-week-old SHR. The addition of Ang II from 10(-12) to 10(-6) mol/L decreased the enzyme activity in SHR to a level similar to that obtained in WKY. The Ang II inhibitory effect was completely reversed by a specific antagonist of AT(2) receptor, PD123319 (10(-8) mol/L) indicating that a system leading to activation of the enzyme in SHR is inhibited by AT(2)-mediated Ang II. Treatment of SHR with losartan for 10 weeks (weeks 4-14) prevents the increase in Na(+)-ATPase activity observed in 14-week-old SHR. These results indicate a correlation between AT(1) receptor activation in SHR and increased ouabain-insensitive Na(+)-ATPase activity. Our results open new possibilities towards our understanding of the pathophysiological mechanisms involved in the increased sodium reabsorption in PT found in essential hypertension.
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Authors | Eugênio P Queiroz-Madeira, Lucienne S Lara, Mira Wengert, Sharon S Landgraf, Janaína D Líbano-Soares, Gisele Zapata-Sudo, Roberto T Sudo, Christina M Takiya, Elaine Gomes-Quintana, Anibal G Lopes, Celso Caruso-Neves |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1798
Issue 3
Pg. 360-6
(Mar 2010)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 19560439
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2009 Elsevier B.V. All rights reserved. |
Chemical References |
- Receptor, Angiotensin, Type 1
- Angiotensin II
- Ouabain
- Sodium-Potassium-Exchanging ATPase
- Losartan
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Topics |
- Angiotensin II
(pharmacology)
- Animals
- Enzyme Activation
(drug effects)
- Hypertension
(enzymology, pathology)
- Kidney Tubules, Proximal
(drug effects, enzymology, pathology)
- Kinetics
- Losartan
(pharmacology)
- Male
- Ouabain
(pharmacology)
- Rats
- Rats, Inbred SHR
- Rats, Inbred WKY
- Receptor, Angiotensin, Type 1
(metabolism)
- Sodium-Potassium-Exchanging ATPase
(metabolism)
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