Expression profiling of the genes responding to zearalenone and its analogues using estrogen-responsive genes.

Abstract

To compare gene expression profiles in response to estrogen or 17beta-estradiol (E(2)) and a mycotoxin, zearalenone (ZEA), and its analogues (collectively termed ZEA compounds), breast cancer MCF-7 cells were treated with 10 nM of E(2) or ZEA compounds including ZEA, alpha-zearalenol, beta-zearalenol, zearalanone, alpha-zearalanol and beta-zearalanol. Expression profiles for 120 estrogen-responsive genes were subjected to cluster and statistical analyses using correlation coefficients or R-values. We found that all of the ZEA compounds stimulated the growth of MCF-7 cells, as much as E(2), and showed similar expression profiles to that of E(2) (R-values ranged from 0.82 to 0.96). The effect of ZEA compounds was likely mediated by estrogen-receptor-dependent Erk1/2-signaling. These results provide clues to understand the mechanism of their estrogen-like action.

• Authors: Meher Parveen, Yun Zhu, Ryoiti Kiyama
• Journal: FEBS letters (FEBS Lett) Vol. 583 Issue 14 Pg. 2377-84 (Jul 21 2009) ISSN: 1873-3468 [Electronic] England
• PMID: 19555691 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Estrogens
• Estrogens, Non-Steroidal
• Receptors, Estrogen
• Estradiol
• Zearalenone
• Extracellular Signal-Regulated MAP Kinases

Topics

• Breast Neoplasms (metabolism)
• Cell Line, Tumor (drug effects)
• Dose-Response Relationship, Drug
• Enzyme Activation
• Estradiol (pharmacology)
• Estrogens (pharmacology)
• Estrogens, Non-Steroidal (chemistry, pharmacology)
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Female
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Molecular Structure
• Oligonucleotide Array Sequence Analysis
• Receptors, Estrogen (metabolism)
• Signal Transduction (drug effects)
• Zearalenone (analogs & derivatives, pharmacology)