Characterization and anti-tumor activities of sulfated polysaccharide SRBPS2a obtained from defatted rice bran.

Abstract

A novel chemically sulfated polysaccharide SRBPS2a with potent anti-tumor activity was derived from defatted rice bran by chlorosulfonic acid-pyridine (CSA-Pyr) method. The average molecular weight of SRBPS2a was 3.5 x 10(5) Da and the degree of sulfation (DS) was 1.29. The Fourier-transform infrared spectra (FT-IR) and 13C NMR spectroscopy analysis revealed that SRBPS2a was mainly consist of beta-(1-->3)-D-galactopyranosyl residues, the sulfate substitution site was on C-2 and C-4 while the side chains were cut off during the sulfated reaction. Furthermore, SRBPS2a exhibited evident growth inhibition on mouse mammary tumor EMT-6 cells both in vitro and in vivo.

• Authors: Li Wang, Hongyu Huang, Yunyu Wei, Xiaoxuan Li, Zhengxing Chen
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 45 Issue 4 Pg. 427-31 (Nov 01 2009) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 19549538 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Polysaccharides
• Sulfates

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacology, therapeutic use)
• Breast Neoplasms (drug therapy)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Female
• Magnetic Resonance Spectroscopy
• Mice
• Molecular Weight
• Oryza (anatomy & histology, chemistry)
• Polysaccharides (chemistry, pharmacology, therapeutic use)
• Spectroscopy, Fourier Transform Infrared
• Structure-Activity Relationship
• Sulfates (chemistry)