Genetic variation of promoter sequence modulates XBP1 expression and genetic risk for vitiligo.

Abstract

Our previous genome-wide linkage analysis identified a susceptibility locus for generalized vitiligo on 22q12. To search for susceptibility genes within the locus, we investigated a biological candidate gene, X-box binding protein 1(XBP1). First, we sequenced all the exons, exon-intron boundaries as well as some 5' and 3' flanking sequences of XBP1 in 319 cases and 294 controls of Chinese Hans. Of the 8 common variants identified, the significant association was observed at rs2269577 (p_(trend) = 0.007, OR = 1.36, 95% CI = 1.09-1.71), a putative regulatory polymorphism within the promoter region of XBP1. We then sequenced the variant in an additional 365 cases and 404 controls and found supporting evidence for the association (p_(trend) = 0.008, OR = 1.31, 95% CI = 1.07-1.59). To further validate the association, we genotyped the variant in another independent sample of 1,402 cases and 1,288 controls, including 94 parent-child trios, and confirmed the association by both case-control analysis (p_(trend) = 0.003, OR = 1.18, 95% CI = 1.06-1.32) and the family-based transmission disequilibrium test (TDT, p = 0.005, OR = 1.93, 95% CI = 1.21-3.07). The analysis of the combined 2,086 cases and 1,986 controls provided highly significant evidence for the association (p_(trend) = 2.94x10(-6), OR = 1.23, 95% CI = 1.13-1.35). Furthermore, we also found suggestive epistatic effect between rs2269577 and HLA-DRB1*07 allele on the development of vitiligo (p = 0.033). Our subsequent functional study showed that the risk-associated C allele of rs2269577 had a stronger promoter activity than the non-risk G allele, and there was an elevated expression of XBP1 in the lesional skins of patients carrying the risk-associated C allele. Therefore, our study has demonstrated that the transcriptional modulation of XBP1 expression by a germ-line regulatory polymorphism has an impact on the development of vitiligo.

• Authors: Yunqing Ren, Sen Yang, Shengxin Xu, Min Gao, Wei Huang, Tianwen Gao, Qiaoyun Fang, Cheng Quan, Chi Zhang, Liangdan Sun, Yanhua Liang, Jianwen Han, Zhimin Wang, Fengyu Zhang, Youwen Zhou, Jianjun Liu, Xuejun Zhang
• Journal: PLoS genetics (PLoS Genet) Vol. 5 Issue 6 Pg. e1000523 (Jun 2009) ISSN: 1553-7404 [Electronic] United States
• PMID: 19543371 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• HLA-DR Antigens
• HLA-DRB1 Chains
• Regulatory Factor X Transcription Factors
• Transcription Factors
• X-Box Binding Protein 1
• XBP1 protein, human

Topics

• Adolescent
• Adult
• Case-Control Studies
• DNA-Binding Proteins (genetics, metabolism)
• Female
• Gene Expression
• Genetic Predisposition to Disease
• Genetic Variation
• HLA-DR Antigens (genetics)
• HLA-DRB1 Chains
• Humans
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Promoter Regions, Genetic
• Regulatory Factor X Transcription Factors
• Transcription Factors (genetics, metabolism)
• Vitiligo (genetics, metabolism)
• X-Box Binding Protein 1
• Young Adult