Assessment of the impact of solvent/detergent treatment on the quality and potency of a whole IgG equine antivenom.

We have evaluated for the first time the impact of a solvent/detergent (S/D) treatment on the quality and in vivo neutralization potency of horse-derived whole IgG antivenom used in the treatment of viperid snake bite envenoming in Central America. The S/D treatment by 1% tri (n-butyl) phosphate (TnBP) - 1% Triton X-45 at 22-25 degrees C was applied either on starting plasma or on purified immunoglobulins. The S/D agents were removed from both fractions by extractions with oil. S/D-treated plasma was subjected to caprylic acid precipitation to purify the immunoglobulins. Products were formulated, sterile-filtered, and filled into 10-mL vials, stored at 5+/-3 degrees C, and subjected to routine quality controls, SDS-PAGE, determination of anti-Bothrops asper venom antibody titre by ELISA, in vivo B. asper venom-neutralization potency tests, and safety test, comparatively with an antivenom manufactured by caprylic acid fractionation without S/D treatment. Results indicate that these conditions of S/D treatment on purified immunoglobulin yielded an antivenom of high turbidity that induced weight loss in animals. In contrast, antivenom fractionated from the S/D-treated plasma had physico-chemical and biological characteristics indistinguishable from those of the non-S/D-treated antivenom. S/D treatment of horse plasma may be considered to increase the viral safety of antivenoms.
AuthorsAlvaro Segura, Guillermo León, Chen-Yao Su, José-María Gutiérrez, Thierry Burnouf
JournalBiologicals : journal of the International Association of Biological Standardization (Biologicals) Vol. 37 Issue 5 Pg. 306-12 (Oct 2009) ISSN: 1095-8320 [Electronic] England
PMID19540775 (Publication Type: Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antivenins
  • Crotalid Venoms
  • Detergents
  • Immunoglobulin G
  • Organophosphates
  • Solvents
  • Octoxynol
  • tributyl phosphate
  • Algorithms
  • Animals
  • Antivenins (adverse effects, drug effects, immunology, therapeutic use)
  • Bothrops (immunology)
  • Crotalid Venoms (immunology)
  • Detergents (pharmacology)
  • Horses (blood, immunology)
  • Immunoglobulin G (chemistry, drug effects)
  • Neutralization Tests (methods, standards)
  • Octoxynol (pharmacology)
  • Organophosphates (pharmacology)
  • Quality Control
  • Snake Bites (drug therapy)
  • Solvents (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: