[Clinical application of avidin-biotin ELISA to detect serum hepatoma-specific gamma-glutamyltransferase in patients with primary hepatic cancer].

Abstract

OBJECTIVE:

To detect serum hepatoma-specific datura stramonium lectin-tightly binding gamma-glutamyl transferase (DSA-GGT) in patients with primary hepatic cancer (PHC) by avidin-biotin ELISA method which was established in our laboratory, and carry on a study of its clinical application.

METHODS:

To detect serum DSA-GGT in 45 healthy control subjects, 58 PHC patients and 203 non-PHC patients (including 36 patients with other tumors and 167 patients with benign liver diseases) with the method was established; meanwhile, AFP was detected by ELISA method.

RESULTS:

38 individuals were DSA-GGT positive in 58 PHC patients, the sensitivity was 65.5%. 18 individuals were DSA-GGT positive in 203 patients without PHC, the specificity was 91.1%. The sensitivity and specificity of AFP in diagnosis of PHC patients was 69.0% and 90.6%, respectively. The sensitivity and specificity of combination of DSA-GGT and AFP was 93.1% and 85.7%, respectively. The average intra-CV and inter-CV of DSA-GGT ELISA was 8.9% and 11.5%, respectively.

CONCLUSION:

The sensitivity and specificity of DSA-GGT ELISA method established in our lab is similar with that of AFP assay and the accuracy is good. Combination of DSA-GGT and AFP may improve the diagnostic sensitivity. The method should be potentially as a new way to improve diagnosis of PHC.

• Authors: Nian-yue Wang, Wei Zhao, Dai Zhang, Yong-cheng Zhang, Mu-hao Duan
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 31 Issue 2 Pg. 114-7 (Feb 2009) ISSN: 0253-3766 [Print] China
• PMID: 19538886 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• alpha-Fetoproteins
• Avidin
• Biotin
• gamma-Glutamyltransferase

Topics

• Adult
• Avidin
• Biotin
• Carcinoma, Hepatocellular (blood, diagnosis, enzymology)
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Humans
• Liver Neoplasms (blood, diagnosis, enzymology)
• Male
• Middle Aged
• Sensitivity and Specificity
• Young Adult
• alpha-Fetoproteins (analysis)
• gamma-Glutamyltransferase (blood)