Expression of HMGA2-LPP and LPP-HMGA2 fusion genes in lipoma: identification of a novel type of LPP-HMGA2 transcript in four cases.

Abstract

BACKGROUND:

In a subset of lipoma, a specific t(3;12)(q27-28;q14-15) chromosomal translocation leads to the fusion of the high mobility group A2 (HMGA2) gene and the lipoma preferred partner (LPP) gene. Although the expression of HMGA2-LPP fusion gene has been reported in lipomas, the reciprocal LPP-HMGA2 fusion gene has rarely been described.

MATERIALS AND METHODS:

Ninety-eight cases of lipoma were analyzed for the possible expression of HMGA2-LPP and LPP-HMGA2 fusion genes using a reverse-transcription polymerase chain reaction method.

RESULTS:

Ten lipomas (10%) revealed both HMGA2-LPP and LPP-HMGA2 fusion transcripts, nine (9%) only HMGA2-LPP, and three (3%) only LPP-HMGA2. DNA sequencing analysis demonstrated that the HMGA2-LPP transcript in 19 lipomas consisted of exons 1-3 of HMGA2 and exons 9-11 of LPP, which was described previously. Out of 13 lipomas with LPP-HMGA2 transcript, 9 were associated with a previously reported LPP-HMGA2 fusion transcript, which fuses exon 8 of LPP to exon 4 of HMGA2, while 4 with a novel type of LPP-HMGA2 fusion transcript, which fuses exon 7 of LPP to exon 4 of HMGA2.

CONCLUSION:

In addition to the HMGA2-LPP fusion gene, the LPP-HMGA2 fusion gene could have some specific roles for lipomagenesis. The biological implications of the expression and the variation of LPP-HMGA2 fusion transcripts need to be elucidated.

• Authors: Takahiro Kubo, Yoshito Matsui, Norifumi Naka, Nobuhito Araki, Tomohiro Goto, Kiminori Yukata, Kenji Endo, Natsuo Yasui, Akira Myoui, Hidehiko Kawabata, Hideki Yoshikawa, Takafumi Ueda
• Journal: Anticancer research (Anticancer Res) Vol. 29 Issue 6 Pg. 2357-60 (Jun 2009) ISSN: 0250-7005 [Print] Greece
• PMID: 19528502 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytoskeletal Proteins
• HMGA2 Protein
• LIM Domain Proteins
• LPP protein, human
• RNA, Messenger
• Recombinant Fusion Proteins

Topics

• Adult
• Aged
• Cytoskeletal Proteins (genetics, metabolism)
• Female
• HMGA2 Protein (genetics, metabolism)
• Humans
• LIM Domain Proteins
• Lipoma (genetics, metabolism)
• Male
• Middle Aged
• RNA, Messenger (genetics, metabolism)
• Recombinant Fusion Proteins (genetics, metabolism)
• Retrospective Studies
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription, Genetic