(-)-Gossypol reduces invasiveness in metastatic prostate cancer cells.

Abstract

BACKGROUND:

Acquisition of metastatic ability by prostatic cancer cells is the most lethal aspect of prostatic cancer progression. (-)-Gossypol, a polyphenolic compound present in cottonseeds, possesses anti-proliferative and proapoptotic effects in various cancer cells.

MATERIALS AND METHODS:

In this study, the differences between MAT-LyLu, rat prostate cancer cells, with a novel isolated subline from metastasized tumors in the lungs of MAT-LyLu-bearing Copenhagen rats (MLL cells) were compared with respect to cell growth and invasion. The effects of (-)-gossypol on cell viability, colony formation, invasive ability and cell migration in MAT-LyLu and MLL cells were also evaluated.

RESULTS:

Results showed that MLL cells displayed higher growth ability, colony formation and aggressive penetration than those of MAT-LyLu cells. MLL cells possess lower protein expression of Bcl-xL and nm23-H1 than those of MAT-LyLu cells, implying differences in invasive ability. Moreover, (-)-gossypol treatment induced a dose-dependent inhibition of invasive activity and cell viability and reduced Bcl-2 and Bcl-xL proteins but induced nm23-H1 protein in both cell lines.

CONCLUSION:

These findings illustrated that (-)-gossypol reduced in vitro invasion of both the parental MAT-LyLu cells and the isolated MLL cells, suggesting that (-)-gossypol might serve as a chemotherapeutic and/or chemopreventive agent.

• Authors: Yi-Wen Huang, Li-Shu Wang, Michael K Dowd, Peter J Wan, Young C Lin
• Journal: Anticancer research (Anticancer Res) Vol. 29 Issue 6 Pg. 2179-88 (Jun 2009) ISSN: 0250-7005 [Print] Greece
• PMID: 19528479 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Contraceptive Agents, Male
• Proto-Oncogene Proteins c-bcl-2
• bcl-X Protein
• Gossypol

Topics

• Animals
• Apoptosis (drug effects)
• Blotting, Western
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Contraceptive Agents, Male (therapeutic use)
• Gossypol (therapeutic use)
• Lung Neoplasms (drug therapy, metabolism, secondary)
• Male
• Neoplasm Invasiveness
• Prostatic Neoplasms (drug therapy, metabolism, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Rats
• bcl-X Protein (metabolism)