Development and characterization of a rat model of chronic obstructive pulmonary disease (COPD) induced by sidestream cigarette smoke.

Cigarette smoke (CS) induced chronic obstructive pulmonary disease (COPD) has been emerging as a great health problem in China. However, lack of appropriate animal model slows down the progress in understanding pathogenesis of the disease. The aim of current study is to establish and evaluate a more adequate rat model of COPD. Study was performed with rats exposed to sidestream cigarette smoke 2h/d and 7d/wk for 2, 4, 6, 8, 10, 12, 24 and 36 wk in a CS chamber (carbon monoxide concentration was 231+/-11ppm). The lung function was determined by using the forced oscillation technique. Pathologic changes were determined by using histological analyses and mucin measurement. Following 36-wk exposure, airway resistance (Raw) and respiratory system elastance (Ers) in CS group rats was elevated by 28.5% and 37.5%, respectively. Up to 4.1-, 2.3- and 1.4-fold increase in the number of neutrophils, macrophages and lymphocytes was observed in the BALF of CS rats. Using quantitative histomorphology techniques, it was found that mean linear intercept (MLI) and mean alveolar airspace (MAA) of CS rats increased by 44.8% and 43.7%, respectively, indicating the occurrence of emphysema. The characteristics of chronic bronchitis including hyperplasia of bronchial epithelial cells, hypersecretion of mucus and development of peribronchial fibrosis were also found in rat lungs. CS group rats showed 43% body weight gain reduction. To conclude, a more adequate sidestream cigarette smoke rat COPD model was established, which will be beneficial for understanding the pathogenesis of the disease and for evaluation of drug effectiveness.
AuthorsHongao Zheng, Yuening Liu, Tian Huang, Zheman Fang, Guishuang Li, Shaoheng He
JournalToxicology letters (Toxicol Lett) Vol. 189 Issue 3 Pg. 225-34 (Sep 28 2009) ISSN: 1879-3169 [Electronic] Netherlands
PMID19524650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Indicators and Reagents
  • Mucins
  • Animals
  • Body Weight (drug effects)
  • Bronchoalveolar Lavage Fluid (cytology)
  • Cell Count
  • Disease Models, Animal
  • Indicators and Reagents
  • Lung (pathology)
  • Male
  • Mucins (metabolism)
  • Pulmonary Disease, Chronic Obstructive (chemically induced, pathology)
  • Pulmonary Fibrosis (pathology)
  • Rats
  • Rats, Wistar
  • Respiratory Function Tests
  • Smoking (pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: