A recombinant-based ELISA evaluating the efficacy of netobimin and albendazole in ruminants with naturally acquired fascioliasis.

Abstract	The therapeutic efficacy of albendazole and netobimin in ruminants with naturally occurring fascioliasis was investigated using a recombinant-based ELISA. The variation in the IgG response against a 2.9-kDa recombinant protein (FhrAPS), termed efficacy index (EI) 1, and the egg-output changes, termed EI 2, were used to evaluate drug efficacy. The values of EI 1 ranged between 0% and 50% in sheep, and between 0% and 30% in cattle after treatment with albendazole and netobimin. Similar EI 2 values were observed in sheep receiving albendazole or netobimin, but the highest values were found in cattle treated with netobimin. The significant reduction in the IgG response to FhrAPS found in this study shows promise in terms of developing alternative methods for evaluating the efficacy of chemotherapy against Fasciola hepatica in grazing ruminants.
Authors	María Sol Arias, José Luis Suárez, G V Hillyer, Iván Francisco, Enrique Calvo, Rita Sánchez-Andrade, Pablo Díaz, Rubén Francisco, Pablo Díez-Baños, Patrocinio Morrondo, Adolfo Paz-Silva
Journal	Veterinary journal (London, England : 1997) (Vet J) Vol. 182 Issue 1 Pg. 73-8 (Oct 2009) ISSN: 1532-2971 [Electronic] England
PMID	19524206 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References	Anthelmintics Antibodies, Helminth Guanidines Helminth Proteins Immunoglobulin G Recombinant Proteins Albendazole netobimin
Topics	Albendazole (therapeutic use) Animals Anthelmintics (therapeutic use) Antibodies, Helminth (blood, immunology) Cattle Cattle Diseases (drug therapy) Enzyme-Linked Immunosorbent Assay (standards, veterinary) Fasciola hepatica (immunology) Fascioliasis (drug therapy, veterinary) Feces (parasitology) Female Guanidines (therapeutic use) Helminth Proteins (immunology) Immunoglobulin G (blood, immunology) Parasite Egg Count (veterinary) Random Allocation Recombinant Proteins (immunology) Sensitivity and Specificity Sheep Sheep Diseases (drug therapy) Treatment Outcome

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