DNMT3B promoter polymorphism and risk of gastric cancer.

Abstract

To investigate the association of single-nucleotide polymorphism (SNP) in DNA methyltransferase 3B (DNMT3B) gene and the risk of gastric cancer (GC), we detected -149C>T and -579G>T in the promoter region of the DNMT3B gene by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing analysis. The DNMT3B genotype was determined in 259 gastric cancer patients and 262 healthy controls that were frequency matched for age and gender. Results showed that individuals with at least one -579G allele were also at significantly decreased risk of gastric cancer [odds ratio (OR), 0.43; 95% confidence interval (CI) 0.26-0.72] compared with those having a -579TT genotype. The -149C>T genotype distribution was irrelevant to the risk of gastric cancer (OR, 1.49; 95% CI, 0.17-17.94) in the studied Chinese population. In addition, data suggested that DNMT3B genetic polymorphism varied among different races, ethnic groups, and geographic areas.

• Authors: Jiabo Hu, Hong Fan, Dongsheng Liu, Shuhong Zhang, Feng Zhang, Huaxi Xu
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 55 Issue 4 Pg. 1011-6 (Apr 2010) ISSN: 1573-2568 [Electronic] United States
• PMID: 19517237 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA (Cytosine-5-)-Methyltransferases
• DNA methyltransferase 3B

Topics

• Adult
• Aged
• Aged, 80 and over
• Alleles
• China
• DNA (Cytosine-5-)-Methyltransferases (genetics)
• DNA Methylation (genetics)
• Female
• Gene Frequency (genetics)
• Genetic Predisposition to Disease (genetics)
• Genetics, Population
• Genotype
• Humans
• Male
• Middle Aged
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length (genetics)
• Polymorphism, Single Nucleotide (genetics)
• Promoter Regions, Genetic (genetics)
• Risk Factors
• Sequence Analysis, DNA
• Stomach Neoplasms (genetics)