Anti-inflammatory effect of coumarins isolated from Corydalis heterocarpa in HT-29 human colon carcinoma cells.

Abstract

We investigated anti-inflammatory effects of two coumarins, columbianetin (A) and libanoridin (B), isolated from Corydalis heterocarpa in lipopolysaccharide (LPS)-stimulated HT-29 human colon carcinoma cells. Treatment with compound B inhibited the protein expression levels of inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) in a dose-dependent manner in LPS-stimulated HT-29 cells, but compound A did not. Also, compound B had a higher inhibitory effect on production of cytokines such as IL-1 beta and TNF-alpha in LPS-stimulated HT-29 human colon carcinoma cells than those of compound A. Furthermore, we confirmed that LPS-induced transcription activity of NF-kappaB was inhibited by compound B. As a result of this study, compound B can be considered as a potential anti-inflammatory agent.

• Authors: Kyong-Hwa Kang, Chang-Suk Kong, Youngwan Seo, Moon-Moo Kim, Se-Kwon Kim
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 47 Issue 8 Pg. 2129-34 (Aug 2009) ISSN: 1873-6351 [Electronic] England
• PMID: 19500635 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Antineoplastic Agents, Phytogenic
• Coloring Agents
• Coumarins
• Furocoumarins
• Inflammation Mediators
• Lipopolysaccharides
• Tetrazolium Salts
• Thiazoles
• libanoridin
• columbianetin
• thiazolyl blue

Topics

• Anti-Inflammatory Agents, Non-Steroidal (isolation & purification, pharmacology)
• Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology)
• Blotting, Western
• Cell Survival (drug effects)
• Coloring Agents
• Corydalis (chemistry)
• Coumarins (isolation & purification, pharmacology)
• Dose-Response Relationship, Drug
• Enzyme-Linked Immunosorbent Assay
• Furocoumarins (isolation & purification, pharmacology)
• HT29 Cells
• Humans
• Inflammation Mediators (analysis)
• Lipopolysaccharides (pharmacology)
• Magnetic Resonance Spectroscopy
• Spectrometry, Mass, Electrospray Ionization
• Spectrophotometry, Ultraviolet
• Tetrazolium Salts
• Thiazoles