Palmatine from Coptidis rhizoma reduces ischemia-reperfusion-mediated acute myocardial injury in the rat.

Abstract

The aim of the present study was to evaluate the protective effect of palmatine, one of active ingredients of Coptidis rhizoma, against myocardial ischemia-reperfusion (I/R) injury is due to its antioxidant and anti-inflammatory action. Adult male rats were subjected to 30 min of ischemia and 6 or 24h of reperfusion. Rats were randomized to receive vehicle or palmatine 1h before reperfusion. Infarct size, myocardial function, and the antioxidant enzyme activity, such as malonaldehyde (MDA), lactate dehydrogenase (LDH), creatine phosphokinase (CK), superoxide dismutase (SOD) and catalase (CAT) were measured. Palmatine significantly improved I/R-induced myocardial dysfunction by increasing the values of the first derivative (+/-dp/dt) of left ventricular pressure and decreased infarct size by 50% (P<0.01 versus vehicle). As expected, palmatine markedly inhibited the increase of LDH, CK, and MDA contents in I/R rat serum, and it also significantly inhibited the decline of the activity of SOD and CAT in I/R cardiac tissues. In addition, COX-2 and iNOS expression in I/R myocardium was significantly reduced. Interestingly, palmatine increased heme oxygenase (HO)-1 induction in human aortic endothelial cells. We concluded that palmatine protects hearts from I/R injury in rats possibly by reducing oxidative stress and modulating inflammatory mediators.

• Authors: Young Min Kim, Yu Mi Ha, Yong Chun Jin, Lian Yu Shi, Yong Soo Lee, Hye Jung Kim, Han Geuk Seo, Jae Soo Choi, Yeong Shik Kim, Sam Sik Kang, Jae Heun Lee, Ki Churl Chang
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 47 Issue 8 Pg. 2097-102 (Aug 2009) ISSN: 1873-6351 [Electronic] England
• PMID: 19497345 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Antioxidants
• Berberine Alkaloids
• Cardiotonic Agents
• Drugs, Chinese Herbal
• Malondialdehyde
• Coptidis rhizoma extract
• L-Lactate Dehydrogenase
• Creatine Kinase
• palmatine

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Antioxidants (pharmacology)
• Berberine Alkaloids (isolation & purification, pharmacology)
• Blotting, Western
• Cardiotonic Agents
• Coptis chinensis
• Creatine Kinase (metabolism)
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Hemodynamics (drug effects)
• L-Lactate Dehydrogenase (metabolism)
• Male
• Malondialdehyde (metabolism)
• Myocardial Infarction (pathology)
• Myocardial Reperfusion Injury (pathology, prevention & control)
• Myocardium (pathology)
• Rats
• Rats, Sprague-Dawley