Abstract | OBJECTIVE: To measure epithelial cell percentages and somatic cell counts (SCCs) in milk and determine whether isoflupredone acetate reduces mammary gland epithelial cell sloughing in cows with acute endotoxin-induced mastitis. ANIMALS: 13 lactating Holstein cows. PROCEDURES: RESULTS: At the 12-hour time point, mean +/- SD percentage of epithelial cells in milk increased from 2.74 +/- 1.93% to 42.11 +/- 36.21% and decreased from 5.73 +/- 4.52% to 5.31 +/- 1.93% in milk from cows with and without mastitis, respectively. Median (range) SCC in milk increased from 195,000 cells/mL (17,000 to 442,000 cells/mL) to 5,437,500 cells/mL (69,000 to 11,036,000 cells/mL) and from 19,000 cells/mL (9,000 to 125,000 cells/mL) to 51,000 cells/mL (10,000 to 835,000 cells/mL) in cows with and without mastitis, respectively. Changes in these variables were significantly greater in mastitis-affected cows. Administration of isoflupredone acetate did not affect epithelial cell percentage or SCC in milk. CONCLUSIONS AND CLINICAL RELEVANCE: During the early phase of endotoxin-induced mastitis in dairy cows, large numbers of epithelial cells were sloughed into the milk. Epithelial cell damage likely precedes an influx of immune cells into affected mammary glands and may contribute to breakdown of the blood-milk barrier.
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Authors | Sarah A Wagner, Douglas E Jones, Michael D Apley |
Journal | American journal of veterinary research
(Am J Vet Res)
Vol. 70
Issue 6
Pg. 796-9
(Jun 2009)
ISSN: 0002-9645 [Print] United States |
PMID | 19496671
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Animals
- Cattle
- Dairying
- Endotoxins
(toxicity)
- Epithelial Cells
(cytology)
- Female
- Mastitis, Bovine
(chemically induced, complications)
- Milk
(cytology)
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