Abstract |
A previous study showed that macelignan extracted from Myristica fragrans has anti-inflammatory properties using hippocampal neuronal and primary microglial cells. Subsequently, a study using animals with chronic lipopolysaccharide (LPS) infusion into the brain showed that oral treatments of macelignan reduced the hippocampal microglial activation and hippocampal-dependent spatial memory impairments induced by LPS. However, the molecular mechanisms responsible for the anti-inflammatory activity of macelignan have not been elucidated in the microglia. Therefore, the present study was conducted to determine if mitogen-activated protein kinase (MAPK) signaling and nuclear factor-kappa B ( NF-kappaB) activities are related to the anti-inflammatory effects of macelignan on LPS-stimulated BV-2 microglial cells. The results show that macelignan suppresses both the phosphorylations of MAPKs and the degradation of inhibitory-kappa B ( IkappaBalpha) and increases of nuclear NF-kappaB in LPS-stimulated BV-2 microglial cells. These results suggest that macelignan has an anti-inflammatory effect on the affected brain through regulation of the inflammation through the MAPK signal pathway.
|
Authors | Jinhua Ma, Yoo Kyeong Hwang, Woo-Hyun Cho, Seol-Heui Han, Jae Kwan Hwang, Jung-Soo Han |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 32
Issue 6
Pg. 1085-90
(Jun 2009)
ISSN: 0918-6158 [Print] Japan |
PMID | 19483320
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Lignans
- Lipopolysaccharides
- NF-kappa B
- macelignan
- Mitogen-Activated Protein Kinases
|
Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(isolation & purification, pharmacology)
- Blotting, Western
- Cell Line
- Cell Nucleus
(drug effects, enzymology, metabolism)
- Cell Survival
(drug effects)
- Cytoplasm
(drug effects, enzymology, metabolism)
- Lignans
(isolation & purification, pharmacology)
- Lipopolysaccharides
- Mice
- Microglia
(drug effects, enzymology, metabolism)
- Mitogen-Activated Protein Kinases
(metabolism)
- Myristica
(chemistry)
- NF-kappa B
(metabolism)
- Phosphorylation
- Signal Transduction
(drug effects)
|