A rapid wound signal activates the systemic synthesis of bioactive jasmonates in Arabidopsis.

Abstract

Jasmonic acid (JA) and its biologically active derivatives (bioactive JAs) perform a critical role in regulating plant responses to wound stress. The perception of bioactive JAs by the F-box protein COI1 triggers the SCF(COI1)/ubiquitin-dependent degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins that repress the expression of JA-response genes. JA is required for many wound-inducible systemic defense responses, but little is known about the role of the hormone in long-distance signal relay between damaged and undamaged leaves. Here, we show that the wounding of Arabidopsis thaliana leaves results in the rapid (<5 min) accumulation of jasmonoyl-l-isoleucine (JA-Ile), the bioactive form of JA, in leaves distal to the wound site. The rapid systemic increase in JA-Ile preceded the onset of early transcriptional responses, and was associated with JAZ degradation. Wound-induced systemic production of JA-Ile required the JA biosynthetic enzyme 12-oxo-phytodienoic acid (OPDA) reductase 3 (OPR3) in undamaged responding leaves, but not in wounded leaves, and was largely dependent on the JA-conjugating enzyme JAR1. Interestingly, the wound-induced synthesis of JA/JA-Ile in systemic leaves was correlated with a rapid decline in OPDA levels. These results are consistent with a model in which a rapidly transmitted wound signal triggers the systemic synthesis of JA, which, upon conversion to JA-Ile, activates the expression of early response genes by the SCF(COI1)/JAZ pathway.

• Authors: Abraham J K Koo, Xiaoli Gao, A Daniel Jones, Gregg A Howe
• Journal: The Plant journal : for cell and molecular biology (Plant J) Vol. 59 Issue 6 Pg. 974-86 (Sep 2009) ISSN: 1365-313X [Electronic] England
• PMID: 19473329 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Arabidopsis Proteins
• COI1 protein, Arabidopsis
• Cyclopentanes
• Fatty Acids, Unsaturated
• Oxylipins
• RNA, Plant
• jasmonoyl-isoleucine
• Isoleucine
• 12-oxophytodienoic acid
• jasmonic acid
• OPR3 protein, Arabidopsis
• Oxidoreductases
• JAR1 protein, Arabidopsis
• Nucleotidyltransferases

Topics

• Adaptation, Physiological
• Arabidopsis (genetics, metabolism)
• Arabidopsis Proteins (metabolism)
• Cyclopentanes (metabolism)
• Fatty Acids, Unsaturated (metabolism)
• Gene Expression Regulation, Plant
• Isoleucine (analogs & derivatives, biosynthesis)
• Nucleotidyltransferases (metabolism)
• Oxidoreductases (metabolism)
• Oxylipins (metabolism)
• Plant Leaves (metabolism)
• Plants, Genetically Modified (genetics, metabolism)
• RNA, Plant (metabolism)
• Signal Transduction