[Effect of sequential intratumoral injection of xenogeneic antigens for immunotherapy in immunized mice bearing S180 tumor].

Abstract

OBJECTIVE:

To investigate the therapeutic effect of sequential intratumoral injection of xenogeneic antigens in immunized tumor-bearing mice.

METHODS:

Sequential intratumoral injection of the xenoantigens was performed in immunized mice bearing S180 tumor. The tumor size changes were observed, and the tumor-infiltrating lymphocytes (TIL) including CD3+CD4+T, CD3+CD8+T, and CD3+CD4+CD25+T lymphocytes were counted with flow cytometry. The concentrations of IL-2 and TNF-alpha in the tumor was measured using ELISA.

RESULTS:

No significant difference was found in the number of CD3+T lymphocytes in the TILs between different groups. After the immunotherapy, the percentages of CD3+CD4+T, CD3+CD8+T and CD3+CD4+CD25+T lymphocytes were 54%, 22% and 2.91%, respectively, with the CD4+/CD8+ ratio of 2.49, significantly different from that in the control group (P<0.05). The concentrations of IL-2 and TNF-alpha were 100.61 pg/ml and 54.114 pg/ml, respectively, significantly different from those in the control group (P<0.05).

CONCLUSION:

Sequential intratumoral injection of heteragenetic antigena can significantly increase the amount of effector cells and cytokines in the micro-environment of the tumor, and decrease the expression of T regulatory.

• Authors: Li-bin Sun, Ji-ren Zhang, Xi-gang Hu, Yan-tao Mao
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 29 Issue 5 Pg. 856-8, 863 (May 2009) ISSN: 1673-4254 [Print] China
• PMID: 19460692 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Heterophile

Topics

• Animals
• Antigens, Heterophile (immunology)
• CD4-CD8 Ratio
• Female
• Immunotherapy (methods)
• Lymphocytes, Tumor-Infiltrating (cytology)
• Male
• Mice
• Random Allocation
• Sarcoma 180 (immunology, therapy)
• Streptococcus (immunology)