Effect of heme oxygenase-1 on the kidney during septic shock in rats.

Abstract

The aim of this study was to clarify the effect of heme oxygenase (HO)-1 on the kidney during septic shock in rats. Eighty healthy and clean Sprague-Dawley rats were randomly divided into 4 groups: control group (group C) , septic shock group (group SS) , lipopolysaccharide plus ZnPP-IX group (group LZ) , and ZnPP-IX (group Z) . The plasma levels of COHb, Cr, and BUN; the urine levels of gamma-GTP, alpha1-MG, RBP, and NAG; and MDA content, SOD activity; HO-1mRNA; HO-2mRNA; HO-1 protein; and HO-2 protein from kidney were measured. The plasma levels of Cr and BUN; the urine levels of gamma-GTP, alpha1-MG, RBP, and NAG; and MDA content from kidney in group SS were obviously higher than in group C and Z (both P < 0.05), respectively, but lower than in group LZ (both P < 0.05), respectively. In contrast, the plasma levels of COHb and SOD activity from kidney in group SS were obviously lower than those in group C and Z (both P < 0.05), respectively, but higher than in group LZ (both P < 0.05), respectively. The HO-1mRNA and HO-1 protein of nephridial tissue in group LZ were obviously higher than in group C and Z, respectively (both P < 0.05), but lower than in group SS, respectively (both P < 0.05). There were no significant differences of HO-2mRNA and HO-2 protein among these 4 groups (both P > 0.05). It is concluded that upregulation of the HO-1 protein might contribute to the protection of kidney during septic shock in rats.

• Authors: Jian-Bo Yu, Fen Zhou, Shang-Long Yao, Zhao-Hui Tang, Man Wang, Hui-Rong Chen
• Journal: Translational research : the journal of laboratory and clinical medicine (Transl Res) Vol. 153 Issue 6 Pg. 283-7 (Jun 2009) ISSN: 1931-5244 [Print] United States
• PMID: 19446282 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipopolysaccharides
• RNA, Messenger
• Malondialdehyde
• Carbon Monoxide
• Heme Oxygenase (Decyclizing)
• Heme Oxygenase-1
• heme oxygenase-2
• Superoxide Dismutase

Topics

• Animals
• Carbon Monoxide (blood)
• Gene Expression Regulation, Enzymologic (physiology)
• Heme Oxygenase (Decyclizing) (genetics, metabolism)
• Heme Oxygenase-1 (genetics, metabolism)
• Kidney (enzymology)
• Kidney Function Tests
• Lipopolysaccharides (pharmacology)
• Male
• Malondialdehyde (blood)
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Shock, Septic (chemically induced, metabolism, physiopathology)
• Superoxide Dismutase (blood)
• Up-Regulation (physiology)