A naturally processed epitope on rotavirus VP7 glycoprotein recognized by HLA-A2.1-restricted cytotoxic CD8+ T cells.

Abstract

Previous studies in mice have shown that CD8(+) T cells can mediate clearance of rotavirus (RV) infection and provide protection from reinfection. Moreover, it has been demonstrated that VP7 can induce a cross-reactive and vigorous specific cytotoxic T-lymphocyte (CTL) response. In this study, a panel of the potential human leukocyte antigen (HLA)-A2.1-restricted CTL epitopes derived from the human RV Wa strain VP7 glycoprotein were screened and assessed using reverse immunogenetics. The specific CTLs induced in vitro by p18-26 (LLNYILKSV)-pulsed autologous dendritic cells from the peripheral blood lymphocytes of healthy HLA-A2.1 donors released interferon-gamma (IFN-gamma) specifically upon stimulation of p18-26, and meanwhile these CTLs lysed p18-26-loaded T2 cells and human RV Wa strain-infected HLA-A2.1(+) Caco-2 cells in an HLA-A2.1-restricted manner. P18-26 was still proved to be immunogenic in vivo in HLA-A2.1/Kb transgenic mice. We propose that the newly identified CTL epitope restricted by HLA-A2.1 could aid in further study of RV-associated infection in humans.

• Authors: Jing Wei, Jintao Li, Xiaoping Zhang, Yan Tang, Jingxue Wang, Yuzhang Wu
• Journal: Viral immunology (Viral Immunol) Vol. 22 Issue 3 Pg. 189-94 (Jun 2009) ISSN: 1557-8976 [Electronic] United States
• PMID: 19435415 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Capsid Proteins
• Epitopes
• HLA-A2 Antigen
• VP7 protein, Rotavirus
• Interferon-gamma

Topics

• Animals
• Antigen Presentation
• Antigens, Viral (immunology, metabolism)
• CD8-Positive T-Lymphocytes (immunology)
• Capsid Proteins (immunology, metabolism)
• Cell Line
• Epitopes (immunology, metabolism)
• HLA-A2 Antigen (immunology, metabolism)
• Humans
• Interferon-gamma (metabolism)
• Leukocytes, Mononuclear (immunology, metabolism)
• Lymphocyte Activation
• Mice
• Rotavirus (immunology)