Huo-Luo-Xiao-Ling Dan modulates antigen-directed immune response in adjuvant-induced inflammation.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

HLXL is a traditional Chinese medicine that has long been used in folk medicine for the treatment of chronic inflammatory diseases. However, the precise immunological mechanisms by which HLXL mediates its anti-inflammatory activity are not fully defined.

AIM OF THE STUDY:

To determine the effects of HLXL on antigen-specific immune parameters in adjuvant-induced inflammation model in the Lewis rat.

MATERIALS AND METHODS:

Rats were fed daily with either HLXL (2.3g/kg) or vehicle (water) beginning 3 days before subcutaneous injection of heat-killed Mycobacterium tuberculosis H37Ra (Mtb), and then continued for another 6 days. After 9 days of Mtb injection, the draining lymph node cells were tested for T cell proliferative and cytokine responses against mycobacterial heat-shock protein 65 (Bhsp65). Moreover, sera were tested for anti-Bhsp65 antibodies and nitric oxide (NO).

RESULTS:

HLXL-treated rats showed reduced T cell proliferative response to Bhsp65 compared to control rats. Furthermore, HLXL suppressed IL-17 response but enhanced IL-10 response without much effect on IFN-gamma. HLXL treatment also reduced the levels of anti-Bhsp65 antibodies but not that of NO.

CONCLUSIONS:

HLXL feeding modulated both the cellular and the humoral immune response to Bhsp65 favoring an anti-inflammatory milieu for the suppression of adjuvant-induced inflammation.

• Authors: Rajesh Rajaiah, David Y-W Lee, Zhongze Ma, Arthur Y Fan, Lixing Lao, Harry H S Fong, Brian M Berman, Kamal D Moudgil
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 123 Issue 1 Pg. 40-4 (May 04 2009) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 19429337 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Antigens
• Cytokines
• Inflammation Mediators

Topics

• Animals
• Antibody Formation
• Antigens (immunology)
• Cytokines (metabolism)
• Disease Models, Animal
• Inflammation (chemically induced, immunology, therapy)
• Inflammation Mediators (metabolism)
• Male
• Medicine, Chinese Traditional
• Rats
• Rats, Inbred Lew