Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.

Abstract

Gliomas are primary brain tumors mainly affecting adults. The cellular origin is unknown. The recent identification of tumor-initiating cells in glioma, which share many similarities with normal neural stem cells, has suggested the cell of origin to be a transformed neural stem cell. In previous studies, using the RCAS/tv-a mouse model, platelet-derived growth factor B (PDGF-B)-induced gliomas have been generated from nestin or glial fibrillary acidic protein-expressing cells, markers of neural stem cells. To investigate if committed glial progenitor cells could be the cell of origin for glioma, we generated the Ctv-a mouse where tumor induction would be restricted to myelinating oligodendrocyte progenitor cells (OPCs) expressing 2',3'-cyclic nucleotide 3'-phosphodiesterase. We showed that PDGF-B transfer to OPCs could induce gliomas with an incidence of 33%. The majority of tumors resembled human WHO grade II oligodendroglioma based on close similarities in histopathology and expression of cellular markers. Thus, with the Ctv-a mouse we have showed that the cell of origin for glioma may be a committed glial progenitor cell.

• Authors: N Lindberg, M Kastemar, T Olofsson, A Smits, L Uhrbom
• Journal: Oncogene (Oncogene) Vol. 28 Issue 23 Pg. 2266-75 (Jun 11 2009) ISSN: 1476-5594 [Electronic] England
• PMID: 19421151 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Avian Proteins
• Platelet-Derived Growth Factor
• Receptors, Virus
• Tva receptor
• Vimentin
• Green Fluorescent Proteins
• Receptor, Platelet-Derived Growth Factor alpha
• Proto-Oncogene Proteins c-akt
• Phosphoric Diester Hydrolases
• 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
• CNP protein, human
• Cnp protein, mouse
• ras Proteins

Topics

• 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
• Animals
• Avian Proteins (genetics)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Chickens
• Genetic Vectors (genetics)
• Glioma (genetics, metabolism, pathology)
• Green Fluorescent Proteins (genetics, metabolism)
• Immunohistochemistry
• Mice
• Mice, Transgenic
• Neoplasms, Experimental (genetics, metabolism, pathology)
• Oligodendroglia (cytology, metabolism)
• Phosphoric Diester Hydrolases (metabolism)
• Platelet-Derived Growth Factor (genetics, metabolism, pharmacology)
• Proto-Oncogene Proteins c-akt (genetics, metabolism)
• Receptor, Platelet-Derived Growth Factor alpha (metabolism)
• Receptors, Virus (genetics)
• Stem Cells (cytology, metabolism)
• Transfection
• Vimentin (metabolism)
• ras Proteins (genetics, metabolism)