Functional single-walled carbon nanotubes based on an integrin alpha v beta 3 monoclonal antibody for highly efficient cancer cell targeting.

Abstract

The application of single-walled carbon nanotubes (SWNTs) in the field of biomedicine is becoming an entirely new and exciting topic. In this study, a novel functional SWNT based on an integrin alpha(v)beta(3) monoclonal antibody was developed and was used for cancer cell targeting in vitro. SWNTs were first modified by phospholipid-bearing polyethylene glycol (PL-PEG). The PL-PEG functionalized SWNTs were then conjugated with protein A. A SWNT-integrin alpha(v)beta(3) monoclonal antibody system (SWNT-PEG-mAb) was thus constructed by conjugating protein A with the fluorescein labeled integrin alpha(v)beta(3) monoclonal antibody. In vitro study revealed that SWNT-PEG-mAb presented a high targeting efficiency on integrin alpha(v)beta(3)-positive U87MG cells with low cellular toxicity, while for integrin alpha(v)beta(3)-negative MCF-7 cells, the system had a low targeting efficiency, indicating that the high targeting to U87MG cells was due to the specific integrin targeting of the monoclonal antibody. In conclusion, SWNT-PEG-mAb developed in this research is a potential candidate for cancer imaging and drug delivery in cancer targeting therapy.

• Authors: Zhongmin Ou, Baoyan Wu, Da Xing, Feifan Zhou, Huiying Wang, Yonghong Tang
• Journal: Nanotechnology (Nanotechnology) Vol. 20 Issue 10 Pg. 105102 (Mar 11 2009) ISSN: 1361-6528 [Electronic] England
• PMID: 19417509 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Drug Carriers
• Integrin alphaVbeta3
• Nanotubes, Carbon

Topics

• Antibodies, Monoclonal (chemistry, immunology, pharmacokinetics, therapeutic use)
• Breast Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Drug Carriers (administration & dosage, chemistry)
• Glioblastoma (metabolism, pathology)
• Humans
• Integrin alphaVbeta3 (chemistry, immunology)
• Nanotubes, Carbon (chemistry)