A
heavy-metal-free luminescent
quantum dot (QD) based on doped
zinc sulfide (ZnS), conjugated with a
cancer-targeting
ligand,
folic acid (FA), is presented as a promising bio-friendly system for targeted
cancer imaging. Doped QDs were prepared by a simple aqueous method at room temperature. X-ray diffraction and transmission electron microscopy studies showed the formation of monodisperse QDs of average size approximately 4 nm with cubic (
sphalerite) crystal structure. Doping of the QDs with metals (Al(3+)), transition metals (Cu(+), Mn(2+)) and halides (F(-)) resulted in multi-color emission with dopant-specific color tunability ranging from blue (480 nm) to red (622 nm). Luminescent centers in doped QDs could be excited using bio-friendly visible light >400 nm by directly populating the dopant centers, leading to bright emission. The cytotoxicity of bare and FA conjugated QDs was tested in vitro using normal lung fibroblast cell line (L929),
folate-receptor-positive (FR+) nasopharyngeal
epidermoid carcinoma cell line (KB), and FR-negative (FR-)
lung cancer cell line (A549). Both bare and FA-conjugated ZnS QDs elicited no apparent toxicity even at high concentrations of approximately 100 microM and 48 h of incubation. In contrast, CdS QDs prepared under identical conditions showed relatively high toxicity even at low concentrations of approximately 0.1 microM and 24 h of incubation. Interaction of FA-QDs with different cell lines showed highly specific attachment of QDs in the FR+
cancer cell line, leaving others unaffected. The bright and stable luminescence of the QDs could be used to image both single
cancer cells and colonies of
cancer cells without affecting their metabolic activity and morphology. Thus, this study presents, for the first time, the use of non-toxic,
Cd-, Te-, Se-, Pb- and Hg-free luminescent QDs for targeted
cancer imaging.