Neuroprotective effect of Buyang Huanwu Decoction on spinal ischemia/reperfusion injury in rats.

Abstract

AIM OF THE STUDY:

The aim of this study was to investigate the protective effect of Buyang Huanwu Decoction, a traditional Chinese medicine formula, on spinal ischemia/reperfusion injury and explore the possible mechanism of the protective effect.

MATERIALS AND METHODS:

The spinal ischemia/reperfusion injury model was conducted in male Sprague-Dawley rats, and 40 g/kg Buyang Huanwu Decoction was administered by introgastric infusion. Motor function of hind limbs and apoptosis index were measured 72 h after reperfusion was started. The expression of thioredoxin and thioredoxin reductase was examined at 6h and at 24h after reperfusion.

RESULTS:

Motor function scores and apoptosis indices were significantly improved in the Buyang Huanwu Decoction group, as compared to the saline-infused control group. Spinal ischemia/reperfusion injury resulted in a decrease in the expression of thioredoxin, while Buyang Huanwu Decoction administration greatly elevated the expression of thioredoxin-1/thioredoxin-2 mRNA and thioredoxin reductase-1/thioredoxin reductase-2 mRNA.

CONCLUSIONS:

Our results suggest that administration of Buyang Huanwu Decoction may reduce spinal ischemia/reperfusion damage. This neuroprotective effect may be mediated, in part, by an increase in the transcription of thioredoxin.

• Authors: Lei Wang, Dian-Ming Jiang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 124 Issue 2 Pg. 219-23 (Jul 15 2009) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 19409971 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Neuroprotective Agents
• RNA, Messenger
• buyang huanwu
• Thioredoxins
• Thioredoxin-Disulfide Reductase

Topics

• Animals
• Apoptosis (drug effects)
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Magnoliopsida
• Male
• Movement (drug effects)
• Neuroprotective Agents (pharmacology, therapeutic use)
• Phytotherapy
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy, metabolism)
• Spinal Cord Ischemia (drug therapy, metabolism)
• Thioredoxin-Disulfide Reductase (metabolism)
• Thioredoxins (metabolism)