[The effect of focal-adhesion micromanipulation in trauma and wound healing].

Abstract

OBJECTIVE:

To explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast.

METHODS:

Micro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides. Skin fibroblasts were cultured on the membrane by self-made biomechanical cell culture for 2 weeks. Morphology observation and cell immunohistochemistry analysis was performed.

RESULTS:

After 2 weeks, the morphology of the fibroblasts was diverse and more compliant. Cell immunohistochemistry analysis found that the expression of integrinbeta1, alpha5 and tensin was dramatically reduced.

CONCLUSIONS:

The morphology and the biological behaviour of the fibroblasts in hypertrophic scar can be changed after micromanipulation of focal adhesion.

• Authors: Yu-zhi Jiang, Jun Wei, Bo Yuan, Ying-kai Liu, Zhi-yong Wang, Kui-fu Ding, Xi-qiao Wang, Ming Tian, Chun-sen Li, Fei Song, Chun Qing, Shu-liang Lu
• Journal: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery (Zhonghua Zheng Xing Wai Ke Za Zhi) Vol. 25 Issue 1 Pg. 57-60 (Jan 2009) ISSN: 1009-4598 [Print] China
• PMID: 19408729 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Cell Culture Techniques
• Cell Growth Processes
• Cells, Cultured
• Cicatrix (surgery)
• Dermis (cytology)
• Female
• Fibroblasts (cytology)
• Focal Adhesions
• Humans
• Immunohistochemistry
• Male
• Wound Healing