Quantitative in vivo studies of soluble simian virus 40 tumor-specific transplantation antigens of the mouse.

Abstract

Quantitative studies have been performed on the immunogenicity of a membrane-bound antigen of a simian virus 40 (SV40) -induced sarcoma in syngeneic BALB/c mice and of subcellular fractions derived from this tumor. The objectives of the investigation were: a) to develop a quantitative in vivo assay of the tumor-specific transplantation antigen (TSTA) and b) to compare the distribution of histocompatibility antigens, H-2, with that of the SV40 TSTA during several fractionation steps. The immunogenicity of the TSTA-containing fractions was assessed from dose-response curves relating tumor size and the amount of protein used for immunization. After digestion of the tumor cell membranes with a limited amount of papain, H-2 as well as TSTA were present in a soluble form. A single immunization with only 2 microng of the solubilized TSTA reduced the tumor size by 70% compared to that in nonimmunized control animals. The results of several fractionation steps suggest that H-2 and the TSTA are not tightly associated in the solubilized immunogenic material.

• Authors: O Henriksen, L W Law, E Appella
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 58 Issue 6 Pg. 1785-8 (Jun 1977) ISSN: 0027-8874 [Print] United States
• PMID: 194045 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antigens, Neoplasm
• Histocompatibility Antigens

Topics

• Animals
• Antigens, Neoplasm (administration & dosage, analysis)
• Cell Membrane (immunology)
• Dose-Response Relationship, Immunologic
• Female
• Histocompatibility Antigens (analysis)
• Mice
• Mice, Inbred BALB C
• Neoplasm Transplantation
• Sarcoma, Experimental (immunology)
• Simian virus 40 (immunology)
• Transplantation, Isogeneic
• Tumor Virus Infections (immunology)