Characteristics of ethylnitrosourea-induced cataracts.

Abstract

PURPOSE:

This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse.

MATERIALS AND METHODS:

Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract.

RESULTS:

A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract.

CONCLUSIONS:

Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.

• Authors: Min-Jung Lee, Jae-Young Kim, Yeun-Jung Kim, Jae-Woo Cho, Kyu-Hyuk Cho, Chang-Woo Song, Han-Sung Jung
• Journal: Current eye research (Curr Eye Res) Vol. 34 Issue 5 Pg. 360-8 (May 2009) ISSN: 1460-2202 [Electronic] England
• PMID: 19401879 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Connexins
• Crystallins
• Eye Proteins
• Proliferating Cell Nuclear Antigen
• Ethylnitrosourea

Topics

• Animals
• Cataract (chemically induced, genetics, metabolism, pathology)
• Connexins (genetics)
• Crystallins (genetics, metabolism)
• DNA Mutational Analysis
• Electrophoresis, Gel, Two-Dimensional
• Ethylnitrosourea
• Eye Proteins (genetics)
• Male
• Mice
• Mice, Inbred BALB C
• Phenotype
• Proliferating Cell Nuclear Antigen (metabolism)
• Protein Array Analysis
• Tissue Distribution